Hajisoltani Razieh, Taghizadeh Mona, Hamblin Michael R, Ramezani Fatemeh
Physiology Research Center, Iran University of Medical Sciences, Tehran, Iran.
Laser Research Centre, Faculty of Health Science, University of Johannesburg, Doornfontein, 2028, South Africa.
Curr Stem Cell Res Ther. 2024 Feb 28. doi: 10.2174/011574888X283713240129095031.
While there is no certain treatment for spinal cord injury (SCI), stem cellbased therapy may be an attractive alternative, but the survival and differentiation of cells in the host tissue are poor. Conditioned medium (CM) has several beneficial effects on cells.
In this meta-analysis study, we examined the effect of CM on SCI treatment.
After searching on MEDLINE, SCOPUS, EMBASE, and Web of Science, first and secondary screening were performed based on title, abstract, and full text. The data were extracted from the included studies, and meta-analysis was performed using STATA.14 software. A standardized mean difference (SMD) with a 95% confidence interval was used to report findings. Quality control and subgroup analysis were also performed.
The results from 52 articles and 61 separate experiments showed that CM had a significantly strong effect on improving motor function after SCI (SMD = 2.58; 95% CI: 2.17 to 2.98; p < 0.001) and also analysis of data from 12 articles demonstrated that CM reduced the expression of GFAP marker (SMD = -4.16; p < 0.0001) compared to SCI group without any treatment. Subgroup analysis showed that treatment with CM of neural stem cells was better than CM of mesenchymal stem cells. It was more effective after a mild lesion than a moderate or severe one. The improvement was more pronounced with <4 weeks than >4 weeks follow-up.
CM had a significant effect in improving motor function after SCI, especially in cases of mild lesions. It has been observed that if CM originates from the neural stem cells, it has a more significant effect than mesenchymal cells.
虽然脊髓损伤（SCI）尚无确切的治疗方法，但基于干细胞的疗法可能是一种有吸引力的替代方案，然而细胞在宿主组织中的存活和分化情况较差。条件培养基（CM）对细胞有多种有益作用。
在这项荟萃分析研究中，我们考察了CM对SCI治疗的效果。
在检索MEDLINE、SCOPUS、EMBASE和科学网之后，基于标题、摘要和全文进行了初次和二次筛选。从纳入的研究中提取数据，并使用STATA.14软件进行荟萃分析。采用具有95%置信区间的标准化均值差（SMD）来报告研究结果。还进行了质量控制和亚组分析。
来自52篇文章和61个独立实验的结果表明，CM对改善SCI后的运动功能有显著强效作用（SMD = 2.58；95% CI：2.17至2.98；p < 0.001），并且对12篇文章的数据进行分析表明，与未接受任何治疗的SCI组相比，CM降低了GFAP标志物的表达（SMD = -4.16；p < 0.0001）。亚组分析表明，用神经干细胞的CM进行治疗优于间充质干细胞的CM。轻度损伤后比中度或重度损伤后更有效。随访<4周时的改善比>4周时更明显。
CM对改善SCI后的运动功能有显著作用，尤其是在轻度损伤病例中。据观察，如果CM源自神经干细胞，其效果比间充质细胞更显著。
