Polat Fatin Rustu, Karaboga Ihsan, Polat Muhammed Semih, Erboga Zeynep, Yilmaz Ahsen, Güzel Savas
Tekirdag Namik Kemal University Medical Faculty, Department of Surgery, Tekirdag, Turkey.
Tekirdag Namik Kemal University, School of Health, Tekirdag, Turkey.
Cell Mol Biol (Noisy-le-grand). 2018 Aug 30;64(11):58-65.
In our study, the effect of hesperetin on inflammatory and oxidative status in trinitrobenzene sulfonic acid (TNBS)-induced experimental colitis model was investigated through different methods. Eighteen Wistar albino male rats were divided in to three groups: Group I (Control, n = 8; 1 ml physiological saline), Group II (Colitis, n = 8; 1 ml TNBS), Group III (Hesperetin, n = 8; 1 ml TNBS and 100 mg/kg hesperetin). Macroscopic and microscopic scores were calculated to determine the damage to the colon at the end of the experiment. Serum tumor necrosis factor-α (TNF-α) and tissue interleukin-6 (IL-6) levels were determined using the ELISA method. Myeloperoxidase (MPO), superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) levels were investigated spectrophotometrically. The TUNEL method was used for the detection of apoptotic cells in the colon tissue. Inducible nitric oxide synthase (iNOS) and nuclear factor-kappa-B (NF-ĸβ) expression in the colon were determined immunohistochemically. Hesperetin administration has shown to significantly reduce levels of MPO, MDA, and proinflammatory agents (TNF-α, IL-6, and NF-ĸβ). It has also been proven to inhibit mucosal apoptosis. This study indicates that hesperetin is protective against TNBS-induced colitis model via antiinflammatory, antioxidant and antiapoptotic effects.
在我们的研究中，通过不同方法研究了橙皮素对三硝基苯磺酸（TNBS）诱导的实验性结肠炎模型中炎症和氧化状态的影响。将18只Wistar白化雄性大鼠分为三组：第一组（对照组，n = 8；1 ml生理盐水），第二组（结肠炎组，n = 8；1 ml TNBS），第三组（橙皮素组，n = 8；1 ml TNBS和100 mg/kg橙皮素）。在实验结束时计算宏观和微观评分以确定结肠的损伤情况。使用ELISA方法测定血清肿瘤坏死因子-α（TNF-α）和组织白细胞介素-6（IL-6）水平。用分光光度法研究髓过氧化物酶（MPO）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和丙二醛（MDA）水平。采用TUNEL法检测结肠组织中的凋亡细胞。用免疫组织化学法测定结肠中诱导型一氧化氮合酶（iNOS）和核因子-κB（NF-κβ）的表达。给予橙皮素已显示出可显著降低MPO、MDA和促炎因子（TNF-α、IL-6和NF-κβ）的水平。还已证明它能抑制黏膜凋亡。本研究表明，橙皮素通过抗炎、抗氧化和抗凋亡作用对TNBS诱导的结肠炎模型具有保护作用。
