Wang X F, Calame K
Cell. 1986 Oct 24;47(2):241-7. doi: 10.1016/0092-8674(86)90446-0.
We have used temperature-sensitive COS cells to design delayed competition experiments in which competition for simian virus 40 (SV40) enhancer factors occurs after enhancer-dependent transcription has been established. The results demonstrate that competition for SV40 enhancer-binding factors has no effect on enhancer-dependent transcription after transcription has been established at the SV40 early promoter. These data show that the enhancer and factors that bind to it are involved in the establishment of stable transcription complexes, although they do not show whether enhancer factors are an integral part of the transcription complexes. Furthermore, the results of delayed competition experiments with a replicating test plasmid are consistent with the possibility that enhancer-dependent stable transcription complexes could be maintained after DNA replication.
我们利用温度敏感型COS细胞设计了延迟竞争实验，其中对猿猴病毒40（SV40）增强子因子的竞争在依赖增强子的转录建立之后发生。结果表明，在SV40早期启动子处转录建立后，对SV40增强子结合因子的竞争对依赖增强子的转录没有影响。这些数据表明，增强子及其结合因子参与了稳定转录复合物的建立，尽管它们并未表明增强子因子是否是转录复合物的一个组成部分。此外，用复制性测试质粒进行的延迟竞争实验结果与DNA复制后依赖增强子的稳定转录复合物能够维持的可能性一致。
