Hoppe U, Hoppe E M, Peskar B M, Peskar B A
FEBS Lett. 1986 Nov 10;208(1):26-30. doi: 10.1016/0014-5793(86)81524-1.
A conjugate of leukotriene (LT) E4 and bovine serum albumin (BSA) was prepared by covalently linking the free amino group of the hapten to the protein using dimethyl pimelindiimidate (DMP) as coupling reagent. Anti-LTE4 antibodies were raised in rabbits immunized with the conjugate. Binding of [3H]LTE4 to the antibodies is inhibited by 50% with 0.63 ng LTE4, while the relative cross-reaction of LTC4 and LTD4 is 46.3% and 12.6%, respectively. Using the radioimmunoassay release of sulfidopeptide-LT (SP-LT) from rat gastric mucosa incubated in vitro was determined after quantitative enzymatic conversion of SP-LT to LTE4. It could be demonstrated that this method is suitable for determination of SP-LT in biological material.
通过使用庚二酸二甲基亚胺酯（DMP）作为偶联试剂，将半抗原的游离氨基与蛋白质共价连接，制备了白三烯（LT）E4与牛血清白蛋白（BSA）的结合物。用该结合物免疫家兔制备抗LTE4抗体。0.63 ng LTE4可使[3H]LTE4与抗体的结合被抑制50%，而LTC4和LTD4的相对交叉反应率分别为46.3%和12.6%。在将硫化肽-LT（SP-LT）定量酶促转化为LTE4后，采用放射免疫分析法测定体外孵育的大鼠胃黏膜中SP-LT的释放。结果表明该方法适用于生物材料中SP-LT的测定。
