通过放射免疫测定法测定人多形核白细胞中白三烯C4的释放。
Release of leukotriene C4 from human polymorphonuclear leucocytes as determined by radioimmunoassay.
作者信息
Aehringhaus U, Wölbling R H, König W, Patrono C, Peskar B M, Peskar B A
出版信息
FEBS Lett. 1982 Sep 6;146(1):111-4. doi: 10.1016/0014-5793(82)80715-1.
Rabbits were immunized with a conjugate of leukotriene (LT) C4 and bovine serum albumin prepared by coupling the single free amino group of the hapten to the protein using gluteraldehyde. Binding of [3H]LTC4 to the antibodies obtained is inhibited by 50% with 1.5 ng LTC4. The relative cross-reaction of LTD4 is 16% and of LTC4-methyl ester 3.6%. The validity of the radioimmunoassay was demonstrated by comparison with bioassay using the isolated guinea pig ileum. Using the radioimmunoassay it could be shown that endogenous LTC4 is released in a dose-dependent manner by human polymorphonuclear leucocytes stimulated with the divalent cation ionophore A23187.
用白三烯（LT）C4与牛血清白蛋白的偶联物免疫兔子，该偶联物是通过戊二醛将半抗原的单个游离氨基与蛋白质偶联制备而成。1.5 ng LTC4可使[3H]LTC4与所得抗体的结合被抑制50%。LTD4的相对交叉反应率为16%，LTC4甲酯的相对交叉反应率为3.6%。通过与使用分离的豚鼠回肠的生物测定法比较，证明了放射免疫测定法的有效性。使用放射免疫测定法可以表明，用二价阳离子载体A23187刺激的人多形核白细胞以剂量依赖方式释放内源性LTC4。
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