Kamholz J, Keyhani J, Gots J S
Gene. 1986;44(1):55-62. doi: 10.1016/0378-1119(86)90042-9.
The purE operon of Escherichia coli has been cloned and localized to a 1.7-kb HpaI fragment. The operon has been further characterized by subcloning into the lac fusion vector, pMC1403, and by the construction of BAL 31-generated deletions. The purE regulation region has been identified by assay of beta-galactosidase produced by pur-lac fusion plasmids and by RNA polymerase binding to end-labelled restriction fragments. Two purE promoters have been identified; one strong that is regulated by purines, the other weaker which is not regulated. The latter may be internal to the purE1 structural gene.
大肠杆菌的purE操纵子已被克隆并定位到一个1.7kb的HpaI片段上。通过亚克隆到lac融合载体pMC1403以及构建BAL 31产生的缺失片段，对该操纵子进行了进一步的表征。通过检测pur-lac融合质粒产生的β-半乳糖苷酶以及RNA聚合酶与末端标记的限制片段的结合，确定了purE调控区。已鉴定出两个purE启动子；一个强启动子受嘌呤调控，另一个较弱的启动子不受调控。后者可能位于purE1结构基因内部。
