a Cátedra de Biología Celular, Histología y Embriología, Instituto de Biología Celular, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba , Córdoba, Argentina.
b Instituto de Investigaciones en Ciencias de la Salud , Universidad Nacional de Córdoba, CONICET , Córdoba , Argentina.
Nutr Cancer. 2018 Oct;70(7):1137-1144. doi: 10.1080/01635581.2018.1497669. Epub 2018 Sep 14.
Retinoic acid (RA) and unsaturated fatty acids (UFA) are proposed as nutritional anticancer agents. Nonetheless, the activity of their combination on human breast cancer needs further study. Our aim was to evaluate this activity on the MCF-7 and ZR-75-1 cell lines treated with 1 µM RA and 50 µM of γ-linoleic (GLA, ω-6), eicosapentaenoic (EPA, ω-3), oleic (OA, ω-9), or eicosatrienoic (ETA, ω-9) acids. The following cellular responses were compared by ANOVA and Fisher test (P < 0.05): fatty acids, E-cadherin, actin (differentiation), conjugated dienes, γ-glutamyltranspeptidase activity (stress), and viability, which were correlated by partial least squares regression. Although both cell lines responded differentially, RA modified unsaturated fatty acids, increased differentiation, reduced γ-glutamyltranspeptidase, and viability. RA differentiating activity on ZR-75-1 was morphologically enhanced by UFA. Stress induction with γ-glutamyltranspeptidase decrease and conjugated dienes was promoted by ETA in MCF-7, and EPA and OA in ZR-75-1. RA-related reduced viability was potentiated by EPA and OA in both lines. GLA was less active. Therefore, unsaturated fatty acids (ω-3/ω-9) potentiated the multitarget retinoic acid activity against these human breast cancer cells.
视黄酸（RA）和不饱和脂肪酸（UFA）被提议作为营养抗癌剂。然而，它们联合应用于人类乳腺癌的活性仍需要进一步研究。我们的目的是评估 MCF-7 和 ZR-75-1 细胞系在 1 µM RA 和 50 µM γ-亚麻酸（GLA，ω-6）、二十碳五烯酸（EPA，ω-3）、油酸（OA，ω-9）或二十碳三烯酸（ETA，ω-9）酸处理下的这种活性。通过方差分析和 Fisher 检验（P < 0.05）比较以下细胞反应：脂肪酸、E-钙黏蛋白、肌动蛋白（分化）、共轭二烯、γ-谷氨酰转肽酶活性（应激）和活力，并用偏最小二乘回归进行相关性分析。尽管两种细胞系的反应不同，但 RA 改变了不饱和脂肪酸，增加了分化，降低了γ-谷氨酰转肽酶和活力。ZR-75-1 上的 RA 分化活性通过 UFA 得到形态增强。在 MCF-7 中，ETA 诱导应激，降低γ-谷氨酰转肽酶和共轭二烯，在 ZR-75-1 中，EPA 和 OA 也是如此。在两种细胞系中，EPA 和 OA 均增强了与 RA 相关的降低活力。GLA 的活性较低。因此，不饱和脂肪酸（ω-3/ω-9）增强了多靶点视黄酸对这些人类乳腺癌细胞的活性。
