Wen Zhixin, Du Xingfan, Meng Nan, Li Yajie, Mi Rui, Li Xuejun, Sun Yongxin, Ma Shuhui, Li Shuying
Dalian Biotechnology Institute, Liaoning Academy of Agricultural Sciences Shida Street No. 2 Dalian 116024 China
RSC Adv. 2019 Feb 13;9(10):5480-5491. doi: 10.1039/c8ra09491h. eCollection 2019 Feb 11.
Silkworms have been reported to promote the growth and production of the stromata of (L.) Link as a parasite insect medium and may improve its metabolites. The effects of Tussah silkmoth pupae (TG group) and rice (RG group) on the metabolic profile of (L.) Link were compared by metabolomics. Meanwhile, the profile of natural (NG group) was also analyzed. The functions of these metabolites from different groups and cordycepin were tested using breast cancer cells and an animal model. 292 metabolites were detected, including 51, 31 and 23 unique metabolites from the TG, RG and NG groups, respectively. The level of 3-deoxyadenosine (cordycepin with anti-tumor activity) was highest in the TG group. Tussah silkmoth pupae induced the biosynthesis of cordycepin and unsaturated fatty acids, which may be beneficial in the prevention of breast cancer. The TG group and cordycepin had significant inhibitory activities on breast cancer cells and in animal models when compared with the two other groups. Tussah silkmoth pupae improved the metabolic profile of (L.) Link, which has more pharmaceutical metabolites than .
据报道，家蚕作为一种寄生昆虫培养基可促进蛹虫草菌核的生长和产生，并可能改善其代谢产物。通过代谢组学比较了柞蚕蛾蛹（TG组）和大米（RG组）对蛹虫草代谢谱的影响。同时，也分析了天然蛹虫草（NG组）的代谢谱。使用乳腺癌细胞和动物模型测试了来自不同组的这些代谢产物以及虫草素的功能。共检测到292种代谢产物，其中TG组、RG组和NG组分别有51种、31种和23种独特的代谢产物。3-脱氧腺苷（具有抗肿瘤活性的虫草素）的水平在TG组中最高。柞蚕蛾蛹诱导了虫草素和不饱和脂肪酸的生物合成，这可能对预防乳腺癌有益。与其他两组相比，TG组和虫草素对乳腺癌细胞和动物模型具有显著的抑制活性。柞蚕蛾蛹改善了蛹虫草的代谢谱，其药用代谢产物比大米培养的蛹虫草更多。
