Rusthoven J J, Adams J, Cheung R K, Dosch H M
Immunol Lett. 1986 Oct;13(4):203-7. doi: 10.1016/0165-2478(86)90056-8.
In response to hypotonic stress, human T and B cells vary in the ability to adjust their volume. Whereas T cells exhibit a regulatory volume decrease (RVD) under these conditions, B cells do not. We studied the ability of peripheral blood-derived natural killer (NK) cells to regulate their volume in this way. Percoll density gradient purified NK subpopulation showed variable RVD which suggested the presence of mixtures of regulatory and non-regulatory cells within these subgroups. Further purification by flow cytometry into Leu 7+ and Leu 11+ cells showed that these NK cells displayed RVD similar to thymocytes but distinct from B cells and more mature T cells. These data support the hypothesis that NK cells may be derived from T cell precursors which, upon differentiation to NK cells, retain the RVD characteristic of pre-T cells. This finding may also be useful in further characterizing neoplastic clones which display NK-like activity but phenotypic heterogeneity.
在低渗应激反应中，人类T细胞和B细胞调节自身体积的能力有所不同。在这些条件下，T细胞会出现调节性体积减小（RVD），而B细胞则不会。我们研究了外周血来源的自然杀伤（NK）细胞以这种方式调节其体积的能力。经Percoll密度梯度纯化的NK亚群显示出可变的RVD，这表明这些亚群中存在调节性细胞和非调节性细胞的混合物。通过流式细胞术进一步纯化到Leu 7 +和Leu 11 +细胞中，结果显示这些NK细胞表现出与胸腺细胞相似但不同于B细胞和更成熟T细胞的RVD。这些数据支持了这样的假设，即NK细胞可能源自T细胞前体，在分化为NK细胞后，保留了前T细胞的RVD特征。这一发现对于进一步表征具有NK样活性但表型异质性的肿瘤克隆也可能有用。
