Zalutsky M R, Noska M A, Gallagher P W
Int J Rad Appl Instrum B. 1986;13(3):269-76. doi: 10.1016/0883-2897(86)90107-8.
Multilamellar liposomes composed of eight different mixtures of distearoylphosphatidylcholine (DSPC) and sphingomyelin (SM) were prepared containing [99mTc]pertechnetate. The in vitro permeability of the liposome preparations in buffer and serum were measured by both dialysis and direct exposure. Liposomes composed of 25-33% SM were least permeable exhibiting leakage half-times of approximately 70 h in buffer and 52 h in serum. Pertechnetate-containing liposomes of three different lipid compositions were injected into mice and the biodistribution of 99mTc activity was determined. All preparations were taken up primarily by the lungs and liver. The half-time for clearance of 99mTc activity ranged from 24.8 to 30.6 h for the liver and 25.3 to 33.0 h for the lungs.
制备了由二硬脂酰磷脂酰胆碱（DSPC）和鞘磷脂（SM）的八种不同混合物组成的多层脂质体，其中含有高锝[99mTc]酸盐。通过透析和直接暴露两种方法测量脂质体制剂在缓冲液和血清中的体外渗透性。由25%-33% SM组成的脂质体渗透性最低，在缓冲液中的泄漏半衰期约为70小时，在血清中为52小时。将三种不同脂质组成的含高锝酸盐脂质体注射到小鼠体内，并测定99mTc活性的生物分布。所有制剂主要被肺和肝脏摄取。肝脏清除99mTc活性的半衰期为24.8至30.6小时，肺为25.3至33.0小时。
