Zalutsky M R, Noska M A, Gallagher P W, Shortkroff S, Sledge C B
Department of Radiology (Nuclear Medicine), Harvard Medical School, Boston, MA 02115.
Int J Rad Appl Instrum B. 1988;15(2):151-6. doi: 10.1016/0883-2897(88)90081-5.
Using [99mTc]pertechnetate as an aqueous space marker, the permeability of liposomes composed of seven different mixtures of distearoylphosphatidylcholine (DSPC) and sphingomyelin (SM) was determined. Liposomes containing 20-33% SM were the least permeable in the presence of rheumatoid synovial fluid. Following injection of 99mTc-containing liposomes into the knee joints of rabbits, retention of 99mTc in the knee was more than 200 times greater than following injection of nonencapsulated [99mTc]pertechnetate. The knee clearance biologic half time of 99mTc with DSPC/SM (4:1) liposomes was 64 h. Most of the activity that had leaked from the knee was not found in extra-articular tissues, suggesting rapid excretion. When DSPC/SM (4:1) liposomes were labeled with 111In(oxine), a knee clearance biologic half time of greater than 1200 h was observed.
以[99mTc]高锝酸盐作为水相空间标志物，测定了由七种不同比例的二硬脂酰磷脂酰胆碱（DSPC）和鞘磷脂（SM）组成的脂质体的通透性。在类风湿性滑液存在的情况下，含有20 - 33% SM的脂质体通透性最低。将含99mTc的脂质体注入兔膝关节后，膝关节中99mTc的保留量比注射未包裹的[99mTc]高锝酸盐后高200多倍。DSPC/SM（4:1）脂质体中99mTc的膝关节清除生物半衰期为64小时。从膝关节渗漏的大部分放射性活性在关节外组织中未被发现，表明排泄迅速。当用111In（氧嗪酸盐）标记DSPC/SM（4:1）脂质体时，观察到膝关节清除生物半衰期大于1200小时。
