蒲公英（Taraxacum formosanum Kitam）的抗氧化特性及其对非小细胞肺癌细胞的抗肿瘤活性。
Antioxidant property of Taraxacum formosanum Kitam and its antitumor activity in non-small-cell lung cancer cells.
机构信息
Department of Food Science, Fu Jen Catholic University, New Taipei 24205, Taiwan.
Graduate Institute of Biomedical Sciences, China Medical University, Taichung 40402, Taiwan.
出版信息
Phytomedicine. 2018 Oct 1;49:1-10. doi: 10.1016/j.phymed.2018.06.011. Epub 2018 Jun 14.
BACKGROUND
Non-small-cell lung cancer (NSCLC) is known to exhibit resistance to various therapeutic agents and become progressively incurable. Taraxacum formosanum is a medicinal Chinese herb that has been clinically used in Taiwan. However, the investigations of the effects of whole plant on lung cancer are limited.
PURPOSE
This study evaluated the in vitro antioxidant, antiproliferative, and antimigration effects of the ethanol extract of T. formosanum (ETF). The possible molecular mechanism underlying its antitumor effects on cultured human NSCLC cell lines was also elucidated.
METHODS
The antioxidant effects of the ETF were determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and Trolox equivalent antioxidant capacity (TEAC) assays, and its antiproliferative and antimigration effects were determined using trypan blue exclusion and wound healing assays, respectively. In addition, changes in the mitogen-activated protein kinase (MAPK) signaling pathway were investigated using Western blot analyses. Various inhibitors were used to determine the roles of the MAPK signaling pathway involved in the molecular mechanism of the ETF.
RESULTS
Our results showed that the ETF exhibited strong reducing power, a high Trolox equivalent antioxidant capacity (TEAC) value, and potent 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging and Fe-chelating abilities. The ETF also exerted antiproliferative and antimigration effects on NSCLC cells in a dose-dependent manner. These effects may be mediated by the inhibitory effects of the ETF on the activation of extracellular signal-regulated kinase.
CONCLUSIONS
This study performed the first pharmacological exploration of T. formosanum. Our results demonstrated the antioxidant and antitumor effects of the ETF on NSCLC cell lines, indicating their potential preventive and therapeutic values for lung cancer.
背景
非小细胞肺癌（NSCLC）已知对各种治疗药物具有耐药性，并逐渐变得无法治愈。蒲公英是一种药用中草药，已在台湾临床使用。然而，对全植物治疗肺癌的研究有限。
目的
本研究评估了蒲公英全草乙醇提取物（ETF）的体外抗氧化、抗增殖和抗迁移作用。还阐明了其对培养的人非小细胞肺癌细胞系的抗肿瘤作用的可能分子机制。
方法
使用 1,1-二苯基-2-苦基肼（DPPH）和 Trolox 等效抗氧化能力（TEAC）测定法测定 ETF 的抗氧化作用，使用台盼蓝排除和划痕愈合测定法分别测定其抗增殖和抗迁移作用。此外，使用 Western blot 分析研究了丝裂原活化蛋白激酶（MAPK）信号通路的变化。使用各种抑制剂来确定 MAPK 信号通路在 ETF 的分子机制中涉及的作用。
结果
我们的结果表明，ETF 表现出强大的还原能力、高 Trolox 等效抗氧化能力（TEAC）值以及有效的 1,1-二苯基-2-苦基肼（DPPH）自由基清除和 Fe 螯合能力。ETF 还以剂量依赖性方式对 NSCLC 细胞表现出抗增殖和抗迁移作用。这些作用可能是通过 ETF 对细胞外信号调节激酶激活的抑制作用介导的。
结论
本研究首次对蒲公英进行了药理学探索。我们的结果表明，ETF 对 NSCLC 细胞系具有抗氧化和抗肿瘤作用，表明其对肺癌具有潜在的预防和治疗价值。
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