环磷酸腺苷相关试剂对牛内皮细胞血管紧张素I转换酶活性的刺激作用。

Stimulation of bovine endothelial cell angiotensin-I-converting enzyme activity by cyclic AMP-related agents.

作者信息

Krulewitz A H, Fanburg B L

出版信息

J Cell Physiol. 1986 Nov;129(2):147-50. doi: 10.1002/jcp.1041290204.

Abstract

Bovine pulmonary artery endothelial cells in culture were used to assess the influence of cyclic nucleotides, isoproterenol (beta adrenergic agonist), and theophylline (phosphodiesterase inhibitor) on angiotensin-I-converting enzyme (ACE) activity of the cells and culture medium. Dibutyryl cAMP (10(-3) M) but not cAMP or dibutyryl cGMP stimulated angiotensin-I-converting enzyme (ACE) activity of cells in culture approximately 50-100% but had little influence on ACE activity of the medium. Theophylline at 10(-3) M concentration produced a three- to fourfold stimulation of both cellular and medium ACE activity. Isoproterenol by itself had no effect on cellular ACE activity but produced a stimulatory effect at 10(-7)-10(-5) M concentration after pretreatment of cells for 24 hr with 10(-4) M theophylline. The results support the concept that ACE activity of endothelial cells is influenced by the cyclic AMP system. ACE activity in cells and that released into medium may be under different regulatory controls.

摘要

培养的牛肺动脉内皮细胞被用于评估环核苷酸、异丙肾上腺素（β肾上腺素能激动剂）和茶碱（磷酸二酯酶抑制剂）对细胞及培养基中血管紧张素I转换酶（ACE）活性的影响。二丁酰环磷腺苷（10⁻³ M）可刺激培养细胞中的血管紧张素I转换酶（ACE）活性增加约50 - 100%，而环磷腺苷或二丁酰环磷鸟苷则无此作用，且二丁酰环磷腺苷对培养基中的ACE活性影响很小。浓度为10⁻³ M的茶碱可使细胞和培养基中的ACE活性提高三到四倍。异丙肾上腺素本身对细胞ACE活性无影响，但在用10⁻⁴ M茶碱预处理细胞24小时后，其在10⁻⁷ - 10⁻⁵ M浓度下产生刺激作用。结果支持内皮细胞ACE活性受环磷腺苷系统影响这一概念。细胞中的ACE活性以及释放到培养基中的ACE活性可能受到不同的调控。