Iwai N, Matsunaga M, Kita T, Tei M, Kawai C
Department of Internal Medicine, Faculty of Medicine, Kyoto University, Japan.
Biochem Biophys Res Commun. 1987 Dec 31;149(3):1179-85. doi: 10.1016/0006-291x(87)90532-8.
Angiotensin converting enzyme (ACE) of vascular endothelial cells is suggested to control vascular wall tonus through the conversion of angiotensin I (AI) to angiotensin II (AII) and the degradation of bradykinin. To obtain more insight into the pathophysiological significance of ACE of vascular endothelial cells, we studied the regulation of ACE produced by cultured human umbilical vein endothelial cells (EC). Phorbol 12-myristate 13-acetate (PMA) increased the cellular and medium ACE activity, accompanied by a marked morphological change in EC. N'-O'-dibutylyladenosine 3';5'-cyclic monophosphate (db-cAMP) increased only the cellular ACE activity and not the medium ACE activity. The effect of isoproterenol with 0.1mM theophylline mimicked that of db-cAMP. These findings suggest that PMA and cAMP-related agents participate in the control of vascular wall tonus through the positive regulation of ACE produced by vascular endothelial cells.
血管内皮细胞的血管紧张素转换酶（ACE）被认为可通过将血管紧张素I（AI）转化为血管紧张素II（AII）以及降解缓激肽来控制血管壁张力。为了更深入了解血管内皮细胞ACE的病理生理意义，我们研究了培养的人脐静脉内皮细胞（EC）产生的ACE的调节机制。佛波醇12-肉豆蔻酸酯13-乙酸酯（PMA）增加了细胞和培养基中的ACE活性，同时EC出现明显的形态变化。N'-O'-二丁酰腺苷3',5'-环磷酸（db-cAMP）仅增加细胞ACE活性，而不增加培养基ACE活性。异丙肾上腺素与0.1mM茶碱共同作用的效果与db-cAMP相似。这些发现表明，PMA和cAMP相关试剂通过对血管内皮细胞产生的ACE进行正向调节，参与血管壁张力的控制。
