Departament de Bioquímica i Biologia Molecular, Facultat de Veterinària, Universitat Autònoma de Barcelona Cerdanyola del Vallès, 08193, Barcelona, Spain.
ITQB NOVA, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras, Portugal.
Sci Rep. 2018 Sep 14;8(1):13850. doi: 10.1038/s41598-018-32241-4.
The effects of different amino acid (AA) supplementations of milk protein-based milk replacers in pre-ruminant calves from 3 days to 7 weeks of age were studied. Animals were divided into 4 groups: Ctrl) Control group fed with milk protein-based milk replacer without supplementation; GP) supplementation with 0.1% glycine and 0.3% proline; FY) supplementation with 0.2% phenylalanine and 0.2% tyrosine; MKT) supplementation with 0.62% lysine, 0.22% methionine and 0.61% threonine. For statistical analysis, t-test was used to compare AA-supplemented animals to the Ctrl group. At week 7, body weight and average daily gain (ADG) were measured and blood samples and skeletal muscle biopsies were taken. Blood biochemistry analytes related to energy metabolism were determined and it was shown that MKT group had higher serum creatinine and higher plasma concentration of three supplemented AAs as well as arginine compared with the Ctrl group. GP group had similar glycine/proline plasma concentration compared with the other groups while in FY group only plasma phenylalanine concentration was higher compared with Control. Although the AA supplementations in the GP and FY groups did not affect average daily gain and metabolic health profile from serum, the metabolome analysis from skeletal muscle biopsy revealed several differences between the GP-FY groups and the Ctrl-MKT groups, suggesting a metabolic adaptation especially in GP and FY groups.
研究了在 3 天至 7 周龄前反刍动物小牛中不同氨基酸（AA）补充牛奶蛋白型代乳粉对牛奶蛋白型代乳粉的影响。动物分为 4 组：Ctrl）对照组，用不含补充剂的牛奶蛋白型代乳粉喂养；GP）补充 0.1%甘氨酸和 0.3%脯氨酸；FY）补充 0.2%苯丙氨酸和 0.2%酪氨酸；MKT）补充 0.62%赖氨酸、0.22%蛋氨酸和 0.61%苏氨酸。为了进行统计分析，使用 t 检验比较了 AA 补充动物与 Ctrl 组。在第 7 周时，测量体重和平均日增重（ADG），并采集血液样本和骨骼肌活检。测定与能量代谢相关的血液生化分析物表明，与 Ctrl 组相比，MKT 组的血清肌酐和三种补充氨基酸（精氨酸）以及血浆浓度更高。GP 组与其他组的甘氨酸/脯氨酸血浆浓度相似，而 FY 组仅血浆苯丙氨酸浓度高于对照。尽管 GP 和 FY 组的 AA 补充并未影响血清中的平均日增重和代谢健康状况，但骨骼肌活检的代谢组分析显示 GP-FY 组与 Ctrl-MKT 组之间存在一些差异，表明存在代谢适应，尤其是在 GP 和 FY 组中。
