用于检测由单纯疱疹病毒及相关猿猴病毒诱导产生的人血清和猿猴血清中群特异性抗体及病毒特异性抗体的酶联免疫吸附测定法。

ELISA for detection of group-common and virus-specific antibodies in human and simian sera induced by herpes simplex and related simian viruses.

作者信息

Katz D, Hilliard J K, Eberle R, Lipper S L

出版信息

J Virol Methods. 1986 Sep;14(2):99-109. doi: 10.1016/0166-0934(86)90040-6.

Abstract

A rapid (3.5 h) enzyme-linked immunosorbent assay (ELISA) was developed for the detection of serum antibodies to herpes simplex virus type 1 (HSV-1) and to two antigenically related monkey viruses, simian agent 8 (SA8) and Herpesvirus simiae (B virus). Crude preparations of detergent solubilized infected cells and similarly treated control mock-infected cells served as antigens for coating wells in microplates. Biotinylated protein A and avidin-conjugated alkaline phosphatase were used to detect antibodies in sera from different species (humans, monkeys and rabbits). Three prototype assays are described with three degrees of specificity. Common or specific determinants on the viral antigens could be assayed in simple competition tests using similar antigen preparations to those coating the wells. The specific assays permitted rapid differential serodiagnosis of antibodies to human and simian herpesviruses.

摘要

开发了一种快速（3.5小时）酶联免疫吸附测定（ELISA），用于检测针对1型单纯疱疹病毒（HSV-1）以及两种抗原相关的猴病毒，即猴因子8（SA8）和猴疱疹病毒（B病毒）的血清抗体。用去污剂溶解的感染细胞粗制品以及经类似处理的对照 mock 感染细胞作为抗原包被微孔板孔。生物素化蛋白A和抗生物素蛋白偶联碱性磷酸酶用于检测来自不同物种（人类、猴子和兔子）血清中的抗体。描述了三种具有不同特异性程度的原型测定法。使用与包被孔的抗原制剂相似的抗原制剂，通过简单的竞争试验可检测病毒抗原上的共同或特异性决定簇。特异性测定法可快速对人类和猴疱疹病毒抗体进行鉴别血清学诊断。