用于检测B型疱疹病毒和SA8病毒抗体的竞争性放射免疫测定法。
Competitive radioimmunoassay to detect antibodies to herpes B virus and SA8 virus.
作者信息
Norcott J P, Brown D W
机构信息
Virus Reference Division, Central Public Health Laboratory, London, United Kingdom.
出版信息
J Clin Microbiol. 1993 Apr;31(4):931-5. doi: 10.1128/jcm.31.4.931-935.1993.
A monoclonal competitive radioimmunoassay (CompRIAm) which detects antibody to herpesvirus simiae (B virus) in monkey and human sera and antibody to SA8 virus in monkey sera but not antibody to herpes simplex virus in human sera is described. Of 232 serum samples from wild-caught cynomolgus monkeys, 117 serum samples were positive when tested by CompRIAm. The results were in close agreement (97.5%) with B virus neutralizing antibody results on the same sera. Sera from 97 wild-caught rhesus monkeys and 92 wild-caught baboons were also tested. The CompRIAm was able to differentiate between sera that had neutralizing antibody to B virus and SA8 virus and those that did not, although the discrimination was not as clear as that in the tests on cynomolgus monkey sera. Sequential sera from two humans with confirmed cases of B virus infection were tested by CompRIAm. B virus antibody was detected in sera from both humans. None of 237 other serum samples from blood donors and patients attending sexually transmitted disease clinics reacted in the CompRIAm.
本文描述了一种单克隆竞争性放射免疫测定法（CompRIAm），该方法可检测猴血清和人血清中抗猴疱疹病毒（B病毒）的抗体以及猴血清中抗SA8病毒的抗体，但不能检测人血清中抗单纯疱疹病毒的抗体。在232份野生食蟹猴血清样本中，经CompRIAm检测，有117份血清样本呈阳性。结果与同一血清样本的B病毒中和抗体结果高度一致（97.5%）。还对97份野生恒河猴血清样本和92份野生狒狒血清样本进行了检测。尽管在食蟹猴血清检测中的区分不如在恒河猴血清检测中那么明显，但CompRIAm能够区分具有B病毒和SA8病毒中和抗体的血清与不具有中和抗体的血清。对两名确诊为B病毒感染的患者的连续血清样本进行了CompRIAm检测。在两名患者的血清中均检测到了B病毒抗体。在237份来自献血者和性传播疾病门诊患者的其他血清样本中，没有一份在CompRIAm检测中出现反应。
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