在单纯疱疹病毒抗体免疫荧光检测中消除人免疫球蛋白G的Fc受体结合。
Elimination of Fc receptor binding of human immunoglobulin G in immunofluorescence assays for herpes simplex virus antibodies.
作者信息
Gallo D
出版信息
J Clin Microbiol. 1986 Oct;24(4):672-4. doi: 10.1128/jcm.24.4.672-674.1986.
Abstract
The binding of human immunoglobulin G by Fc receptors in herpes simplex virus (HSV)-infected cells can cause false-positive interpretations in the immunofluorescence test for HSV antibody. When the infected cell smears were treated with 10% glacial acetic acid for 5 min and rinsed in phosphate-buffered saline before the immunofluorescence test was performed, the Fc receptors were completely inactivated, resulting in a reliable method for HSV antibody detection.
摘要
单纯疱疹病毒（HSV）感染细胞中的Fc受体与人免疫球蛋白G的结合，可能会在HSV抗体的免疫荧光检测中导致假阳性结果。在进行免疫荧光检测之前，用10%的冰醋酸处理感染细胞涂片5分钟，然后用磷酸盐缓冲盐水冲洗，Fc受体就会完全失活，从而得到一种可靠的HSV抗体检测方法。
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