Dudylina Arina L, Ivanova Marina V, Shumaev Konstantin B, Ruuge Enno K
Faculty of Physics, Lomonosov Moscow State University, Moscow, Russia.
National Medical Research Centre for Cardiology, Moscow, Russia.
Cell Biochem Biophys. 2019 Mar;77(1):99-107. doi: 10.1007/s12013-018-0857-2. Epub 2018 Sep 14.
Since mitochondria are the main cellular source of reactive oxygen species, it is important to study the effect of dietary phenolic compounds on the level of ROS in these organelles. Using the EPR spectroscopy and TIRON probe, the ability of the investigated phenols (quercetin, rutin, caffeic acid, curcumin, and resveratrol) to scavenge superoxide anion radicals generated by isolated heart mitochondria of Wistar rats under variable oxygen partial pressure was studied. It was shown that during a 10 min incubation, caffeic acid in concentrations of 10-500 μM most effectively scavenged superoxide radicals formed in the complex III of the mitochondrial respiratory chain. A comparable antioxidant effect of rutin under these experimental conditions was observed at higher concentrations of 1-10 mM. The antioxidant activity of quercetin in the concentration range of 10-500 μM during the first minutes of incubation was higher than that of caffeic acid. Of the phenolic compounds studied, curcumin had the least effect on the superoxide radicals.
由于线粒体是细胞内活性氧的主要来源，因此研究膳食酚类化合物对这些细胞器中活性氧水平的影响非常重要。使用电子顺磁共振光谱法和TIRON探针，研究了所研究的酚类物质（槲皮素、芦丁、咖啡酸、姜黄素和白藜芦醇）在不同氧分压下清除Wistar大鼠离体心脏线粒体产生的超氧阴离子自由基的能力。结果表明，在10分钟的孵育过程中，浓度为10 - 500μM的咖啡酸最有效地清除了线粒体呼吸链复合物III中形成的超氧自由基。在1 - 10 mM的较高浓度下，观察到芦丁在这些实验条件下具有类似的抗氧化作用。在孵育的最初几分钟内，浓度范围为10 - 500μM的槲皮素的抗氧化活性高于咖啡酸。在所研究的酚类化合物中，姜黄素对超氧自由基的影响最小。
