Bermúdez-Oria Alejandra, Godoy Eugenia, Pérez Virginia, Musci Ferrari Camila, Donato Martin, Fernández-Bolaños Juan, Zaobornyj Tamara, D'Annunzio Verónica
Department of Food Phytochemistry, Instituto de la Grasa (Spanish National Research Council, CSIC), Ctra. de Utrera km. 1, Pablo de Olavide University Campus, Building 46, 41013 Seville, Spain.
Facultad de Farmacia y Bioquímica, Instituto de Bioquimica y Medicina Prof. Alberto Boveris (IBIMOL, UBA-CONICET), Universidad de Buenos Aires, Buenos Aires 1113, Argentina.
Antioxidants (Basel). 2025 Jun 28;14(7):803. doi: 10.3390/antiox14070803.
Cardiovascular diseases remain the leading cause of death worldwide, highlighting the urgent need for novel therapeutic strategies. The Mediterranean diet is renowned for its cardiovascular benefits, largely attributed to extra virgin olive oil (EVOO) and its phenolic compounds, particularly hydroxytyrosol (HT). HT, a potent antioxidant and anti-inflammatory agent, has demonstrated significant therapeutic potential in mitigating myocardial damage following acute myocardial infarction (AMI). However, there is a notable lack of published evidence regarding the effects of HT administration in the context of acute ischemia/reperfusion (I/R) injury, making this study a novel contribution to the field. This study aimed to evaluate the cardioprotective effects of HT using the Langendorff technique in an isolated mouse heart ischemia/reperfusion (I/R) model. Mice were administered a single intraperitoneal dose of HT (10 mg/kg) 24 h prior to the I/R protocols, and parameters such as the infarct size, mitochondrial function, and redox balance were assessed. The results revealed a remarkable 57% reduction in infarct size in HT-treated mice compared to untreated controls. HT treatment also improved mitochondrial bioenergetics, as evidenced by the increased membrane potential (ΔΨm), enhanced oxygen consumption, and reduced hydrogen peroxide (HO) production. Furthermore, HT restored the activity of the mitochondrial respiratory complexes, notably Complex I, even under I/R conditions. These findings highlight the efficacy of HT in reducing oxidative stress and preserving mitochondrial function, critical factors in cardiac disease. In conclusion, HT emerges as a promising therapeutic agent for ischemic heart disease, demonstrating both preventive and restorative potential. Future research should explore its clinical applicability to advance cardiovascular disease management.
心血管疾病仍然是全球主要的死亡原因，这凸显了对新型治疗策略的迫切需求。地中海饮食因其对心血管有益而闻名，这在很大程度上归因于特级初榨橄榄油（EVOO）及其酚类化合物，特别是羟基酪醇（HT）。HT是一种有效的抗氧化剂和抗炎剂，已显示出在减轻急性心肌梗死（AMI）后心肌损伤方面具有显著的治疗潜力。然而，关于在急性缺血/再灌注（I/R）损伤背景下给予HT的效果，目前明显缺乏已发表的证据，因此本研究为该领域做出了新的贡献。本研究旨在使用Langendorff技术在分离的小鼠心脏缺血/再灌注（I/R）模型中评估HT的心脏保护作用。在进行I/R实验方案前24小时，给小鼠腹腔注射单次剂量的HT（10mg/kg），并评估梗死面积、线粒体功能和氧化还原平衡等参数。结果显示，与未治疗的对照组相比，HT治疗组小鼠的梗死面积显著减少了57%。HT治疗还改善了线粒体生物能量学，表现为膜电位（ΔΨm）增加、氧气消耗增强和过氧化氢（HO）生成减少。此外，即使在I/R条件下，HT也恢复了线粒体呼吸复合物的活性，尤其是复合物I的活性。这些发现突出了HT在减少氧化应激和保护线粒体功能方面的功效，而氧化应激和线粒体功能是心脏病的关键因素。总之，HT成为一种有前途的缺血性心脏病治疗药物，具有预防和恢复的潜力。未来的研究应探索其临床适用性，以推进心血管疾病的管理。
