Amist Nimisha, Singh N B, Yadav Kavita, Singh S C, Pandey J K
Plant Physiology Laboratory, Department of Botany, University of Allahabad, Allahabad, 211002, India.
Plant Physiology Laboratory, Department of Botany, University of Allahabad, Allahabad, 211002, India.
J Biotechnol. 2017 Jul 20;254:1-8. doi: 10.1016/j.jbiotec.2017.06.002. Epub 2017 Jun 8.
In the present study, we compared the effects of Al ions and alumina (AlO) nanoparticles (NPs) on growth and biochemical parameters of cabbage seedlings. The seedlings were treated with A=698.8μM Alions and A=101.8μM, A=253.8μM, A=507.7μM, A=1.02mM, A=2.17mM NPs of AlO in 700mL Hoagland solution. The untreated seedlings were taken as control. It was observed that aluminium ions were phytotoxic and adversely affected seedling growth and biochemical parameters of the test crop with stunting of the stem growth, while lower doses of alumina NPs enhanced seedling growth, pigments, sugar and protein contents of cabbage (Brassica oleracea var. capitata) seedlings. Higher doses of alumina NPs adversely affected biochemical parameters and nitrate reductase activities of the treated seedlings. Alumina NPs induced activities of antioxidant enzymes viz. SOD, CAT and POX. Antioxidant enzyme activities increased under all treatments with maximum increase in the seedlings treated with aluminium ions and higher concentrations of alumina NPs. The lower amount of alumina NPs buttressed the metabolic processes of the test crop and appeared to mitigate the phytotoxic effects of aluminium ions.
在本研究中，我们比较了铝离子和氧化铝（AlO）纳米颗粒（NPs）对甘蓝幼苗生长和生化参数的影响。将幼苗置于700毫升霍格兰溶液中，分别用浓度为698.8μM的铝离子以及浓度为101.8μM、253.8μM、507.7μM、1.02mM、2.17mM的AlO纳米颗粒进行处理。未处理的幼苗作为对照。结果观察到，铝离子具有植物毒性，会对受试作物的幼苗生长和生化参数产生不利影响，导致茎生长受阻，而较低剂量的氧化铝纳米颗粒则促进了甘蓝（Brassica oleracea var. capitata）幼苗的生长、色素、糖分和蛋白质含量。较高剂量的氧化铝纳米颗粒对处理过的幼苗的生化参数和硝酸还原酶活性产生了不利影响。氧化铝纳米颗粒诱导了抗氧化酶即超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和过氧化物酶（POX）的活性。在所有处理下，抗氧化酶活性均有所增加，其中用铝离子和较高浓度氧化铝纳米颗粒处理的幼苗中增加幅度最大。较低量的氧化铝纳米颗粒支持了受试作物的代谢过程，并且似乎减轻了铝离子的植物毒性作用。
