Dal Canto M C, Barbano R L, Jubelt B
J Neuropathol Exp Neurol. 1986 Nov;45(6):613-8. doi: 10.1097/00005072-198611000-00001.
Ultrastructural immunohistochemistry was used to localize type 2 human poliovirus (HPV 2) during virulent infection of mice caused by the Lansing strain. In the spinal cord, immune-reaction product was exclusively localized within neurons and their processes. The absence of viral antigen in glial, endothelial and inflammatory cells further supports the strict neuronotropicity of HPV. In addition, viral antigen and virus-like particles were localized in synaptic complexes and axons, including preterminal axons. This clear demonstration of HPV in neuronal cell bodies, their axons, and synaptic elements strongly supports the hypothesis of HPV dissemination in the central nervous system via axonal transport.
运用超微结构免疫组织化学技术，在兰辛株引发的小鼠强毒感染期间对2型人脊髓灰质炎病毒（HPV 2）进行定位。在脊髓中，免疫反应产物仅定位于神经元及其突起内。神经胶质细胞、内皮细胞和炎性细胞中不存在病毒抗原，这进一步支持了HPV严格的嗜神经性。此外，病毒抗原和病毒样颗粒定位于突触复合体和轴突，包括终末前轴突。HPV在神经元细胞体、其轴突和突触元件中的这种清晰显示，有力地支持了HPV通过轴突运输在中枢神经系统中传播的假说。
