Inagami T, Nakamaru M, Pandey K N, Naruse M, Naruse K, Misono K, Okamura T, Kawamura M
J Hypertens Suppl. 1986 Oct;4(4):S11-6.
The enzyme renin has been purified and characterized by structural analysis. Pure renin protein was used to produce a specific antibody to renin, which was useful in demonstrating the presence of a specific renin in many tissues other than kidney. In these cells angiotensins I and II and angiotensin converting enzyme were found to coexist with renin by immunohistochemical studies and by the direct determination with cultured cells. Studies with these cells indicated the local production of renin, angiotensinogen and angiotensins in these cells. Angiotensin II produced in the cultured cells was secreted to the outside of the cells while more than 95% renin remained within the cells. Secretion of angiotensin II from the angiotensin producing cells was demonstrated with perfused mesenteric artery. The secretion was stimulated by the adrenergic beta-agonist isoproterenol in a dose-dependent manner and specifically inhibited by a beta 2-antagonist. Angiotensin II secreted from the vascular bed by the beta 2-adrenoceptor stimulation acts locally to facilitate norepinephrine release. These studies demonstrate local production and secretion of angiotensin II and define its physiological role.
肾素酶已通过结构分析进行了纯化和特性鉴定。使用纯肾素蛋白制备了肾素特异性抗体，该抗体有助于证明除肾脏外的许多组织中存在特异性肾素。通过免疫组织化学研究以及对培养细胞的直接测定发现，在这些细胞中血管紧张素I和II以及血管紧张素转换酶与肾素共存。对这些细胞的研究表明，这些细胞中存在肾素、血管紧张素原和血管紧张素的局部产生。培养细胞中产生的血管紧张素II分泌到细胞外，而超过95%的肾素保留在细胞内。通过灌注肠系膜动脉证明了血管紧张素产生细胞分泌血管紧张素II。该分泌受到肾上腺素能β激动剂异丙肾上腺素的剂量依赖性刺激，并被β2拮抗剂特异性抑制。β2肾上腺素能受体刺激从血管床分泌的血管紧张素II在局部起作用，促进去甲肾上腺素释放。这些研究证明了血管紧张素II的局部产生和分泌，并确定了其生理作用。
