Inagami T, Mizuno K, Nakamaru M, Pandey K N, Naruse M, Naruse K, Misono K, Okamura T, Kawamura M, Higashimori K
Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.
Cardiovasc Drugs Ther. 1988 Nov;2(4):453-8. doi: 10.1007/BF00051182.
The enzyme renin has been purified and characterized by structural analysis. Pure renin protein was used to produce a specific antibody to renin, which was useful in demonstrating the presence of a specific renin in many tissues other than kidney. Further, in these cells angiotensins I and II and converting enzyme all were found to coexist with renin by immunohistochemical studies, indicating the local production of renin, angiotensinogen and angiotensins in these cells. Angiotensin II produced in the cultured cells was secreted to the outside of the cells. Secretion of angiotensin II from the angiotensin-producing cells was demonstrated with perfused mesenteric artery. The secretion of angiotensin II from the vascular beds was inhibited by converting enzyme inhibitors, and was stimulated by the adrenergic beta-agonist isoproterenol. These studies demonstrate local production and controlled secretion of angiotensin II and define its physiologic role.
肾素酶已通过结构分析进行了纯化和表征。使用纯肾素蛋白制备了针对肾素的特异性抗体，该抗体有助于证明除肾脏外的许多组织中存在特异性肾素。此外，通过免疫组织化学研究发现，在这些细胞中血管紧张素I、II和转化酶均与肾素共存，表明这些细胞中存在肾素、血管紧张素原和血管紧张素的局部产生。培养细胞中产生的血管紧张素II分泌到细胞外。通过灌注肠系膜动脉证明了血管紧张素产生细胞分泌血管紧张素II。血管紧张素II从血管床的分泌受到转化酶抑制剂的抑制，并受到肾上腺素能β激动剂异丙肾上腺素的刺激。这些研究证明了血管紧张素II的局部产生和受控分泌，并确定了其生理作用。
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