血清层粘连蛋白-2 水平升高与食管鳞状细胞癌相关。

Elevated levels of serum nidogen-2 in esophageal squamous cell carcinoma.

机构信息

Department of Clinical Oncology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.

Department of Surgery, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.

出版信息

Cancer Biomark. 2018 Feb 14;21(3):583-590. doi: 10.3233/CBM-170484.

DOI:10.3233/CBM-170484

PMID:29278876

Abstract

BACKGROUND

Nidogen-2 (NID2), a secretory basement membrane protein, has been implicated as a potential biomarker in ovarian cancer and hepatocellular carcinoma.

OBJECTIVE

In this study, we aimed to investigate the utility of detecting serum NID2 levels for identification of esophageal squamous cell carcinoma (ESCC) patients and prediction of poor survival outcome.

METHODS

Using an in-house NID2 enzyme-linked immunosorbent assay (ELISA), serum samples from 101 ESCC patients and 50 healthy controls were screened for their NID2 levels.

RESULTS

The serum NID2 levels in ESCC patients (median 24.4 μg/L) are significantly higher (p= 4.3e-09) than that of the healthy controls (median 15.85 μg/L). The receiver operating characteristic (ROC) curve demonstrated an area under the curve of 0.756. At the threshold of 17.95 μg/L, the sensitivity and specificity achieved are 0.76 and 0.63, respectively. Kaplan-Meier survival analysis revealed that patients with high serum NID2 levels (⩾ 32.6 μg/L) have significantly higher risk of death (HR = 1.984, 95% CI: 1.175-3.349; log-rank p-value = 0.012) compared to those with low serum NID2 levels (< 20.0 μg/L).

CONCLUSIONS

In conclusion, we show that detecting the elevation of serum NID2 levels has potential diagnostic and prognostic value for ESCC patients.

摘要

背景

层粘连蛋白-2（NID2）是一种分泌型基底膜蛋白，已被认为是卵巢癌和肝细胞癌的潜在生物标志物。

目的

本研究旨在探讨检测血清 NID2 水平对识别食管鳞状细胞癌（ESCC）患者和预测不良生存结局的应用价值。

方法

使用内部 NID2 酶联免疫吸附测定（ELISA）法，筛选了 101 例 ESCC 患者和 50 例健康对照者的血清样本，以检测其 NID2 水平。

结果

ESCC 患者的血清 NID2 水平（中位数 24.4μg/L）明显高于健康对照组（中位数 15.85μg/L）（p=4.3e-09）。受试者工作特征（ROC）曲线分析显示曲线下面积为 0.756。在 17.95μg/L 的阈值下，灵敏度和特异性分别为 0.76 和 0.63。Kaplan-Meier 生存分析显示，血清 NID2 水平较高（⩾32.6μg/L）的患者死亡风险显著更高（HR=1.984，95%CI：1.175-3.349；对数秩检验 p 值=0.012），与血清 NID2 水平较低（<20.0μg/L）的患者相比。