相关枯草芽孢杆菌噬菌体SPP1、41c、rho 15和SF6的基因组结构
Genomic organization of the related Bacillus subtilis bacteriophages SPP1, 41c, rho 15, and SF6.
作者信息
Santos M A, Almeida J, de Lencastre H, Morelli G, Kamke M, Trautner T A
出版信息
J Virol. 1986 Nov;60(2):702-7. doi: 10.1128/JVI.60.2.702-707.1986.
Abstract
The genomes of the related virulent Bacillus subtilis bacteriophages SPP1, 41c, rho 15, and SF6 are partially circularly permuted and terminally redundant. Heteroduplex molecules were produced with various combinations of these DNAs. Their electron-microscopic analyses showed a consistent pattern of homologous and heterologous regions of DNA. Restriction maps of the phage DNAs were established. A comparison of these maps showed a pattern of conserved and variable DNAs compatible with the electron-microscopic analyses. In all phage genomes, regions specifying early and late functions were conserved. In each phage genome, such regions were separated by short segments of heterologous DNA characteristic for each phage.
摘要
相关的毒性枯草芽孢杆菌噬菌体SPP1、41c、rho 15和SF6的基因组部分呈环状排列且末端冗余。用这些DNA的各种组合产生了异源双链分子。它们的电子显微镜分析显示了DNA同源和异源区域的一致模式。建立了噬菌体DNA的限制性图谱。这些图谱的比较显示了与电子显微镜分析相符的保守和可变DNA模式。在所有噬菌体基因组中，指定早期和晚期功能的区域是保守的。在每个噬菌体基因组中，这些区域被每个噬菌体特有的异源DNA短片段隔开。
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