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枯草芽孢杆菌噬菌体SSP1的基因组:DNA分子的拓扑结构

The genome of B. subtilis phage SSP1: the topology of DNA molecules.

作者信息

Morelli G, Fisseau C, Behrens B, Trautner T A, Luh J, Ratcliff S W, Allison D P, Ganesan A T

出版信息

Mol Gen Genet. 1979 Jan 10;168(2):153-61. doi: 10.1007/BF00431441.

DOI:10.1007/BF00431441
PMID:109737
Abstract

DNA molecules of B. subtilis phage SPP1 exhibit terminal redundancy and are partially circularly permuted. This was established by the hybridization of selected EcoRI restriction fragments to single strands of SPP1 DNA and by an analysis of the distribution of denaturation loops in partially denatured SPP1 DNA molecules. Deletions in SSP1 DNA are not compensated by an increase in terminally repetitious DNA. This finding, which is unique to SPP1, is discussed in terms of a modification of the Streisinger/Botstein model of phage maturation.

摘要

枯草芽孢杆菌噬菌体SPP1的DNA分子表现出末端冗余且部分呈环状排列。这是通过将选定的EcoRI限制性片段与SPP1 DNA单链杂交以及分析部分变性的SPP1 DNA分子中变性环的分布来确定的。SPP1 DNA中的缺失不会通过末端重复DNA的增加来补偿。这一SPP1特有的发现,根据噬菌体成熟的斯特赖辛格/博特斯坦模型的修正进行了讨论。

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本文引用的文献

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Alignment of partial denaturation maps of circularly permuted DNA by computer.通过计算机对环状排列DNA的部分变性图谱进行比对。
J Mol Biol. 1974 Jan 5;85(4):528-32. doi: 10.1016/0022-2836(74)90313-1.
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Genome of Bacillus subtilis Bacteriophage SPP1: Structure and Nucleotide Sequence of pac, the Origin of DNA Packaging.枯草芽孢杆菌噬菌体SPP1的基因组:DNA包装起始位点pac的结构与核苷酸序列
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Promoter sites in the genome of B. subtilis phage SPP1.枯草芽孢杆菌噬菌体SPP1基因组中的启动子位点。
Mol Gen Genet. 1981;181(4):518-21. doi: 10.1007/BF00428745.
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Maturation of bacteriophage SPPI DNA: limited precision in the sizing of mature bacteriophage genomes.噬菌体SPPI DNA的成熟:成熟噬菌体基因组大小确定中的有限精度。
J Virol. 1981 Feb;37(2):832-5. doi: 10.1128/JVI.37.2.832-835.1981.
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Membrane binding of bacteriophage SPP1 DNA.噬菌体SPP1 DNA的膜结合
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10
The effect of trimethylpsoralen--crosslinks on entry of donor DNA in transformation and transfection of Bacillus subtilis.三甲补骨脂素交联对供体DNA进入枯草芽孢杆菌转化和转染过程的影响。
Mol Gen Genet. 1980;179(1):1-11. doi: 10.1007/BF00268439.