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噬菌体T4完整rIIB基因的克隆及其中间启动子的一些观察

Cloning the complete rIIB gene of bacteriophage T4 and some observations concerning its middle promoters.

作者信息

Shinedling S, Walker L T, Gold L

出版信息

J Virol. 1986 Nov;60(2):787-92. doi: 10.1128/JVI.60.2.787-792.1986.

DOI:10.1128/JVI.60.2.787-792.1986
PMID:3022011
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC288959/
Abstract

We cloned the intact T4 rIIB gene by joining plasmids carrying gene fragments. rIIB was expressed at a low level under control of the lac promoter, and the clone complemented rIIB mutants. We suspect that earlier attempts to clone the intact gene were unsuccessful because of transcription from T4 middle-mode promoters. These promoters are silent early in infection but are recognized when resident on a plasmid in an uninfected cell.

摘要

我们通过连接携带基因片段的质粒克隆了完整的T4 rIIB基因。rIIB在乳糖启动子的控制下低水平表达，并且该克隆对rIIB突变体具有互补作用。我们怀疑早期克隆完整基因的尝试未成功是由于T4中期模式启动子的转录。这些启动子在感染早期是沉默的，但当存在于未感染细胞的质粒上时会被识别。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d582/288959/5486fe4602f3/jvirol00168-0464-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d582/288959/a33f1e80017e/jvirol00168-0463-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d582/288959/5486fe4602f3/jvirol00168-0464-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d582/288959/a33f1e80017e/jvirol00168-0463-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d582/288959/5486fe4602f3/jvirol00168-0464-a.jpg

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Cloning the complete rIIB gene of bacteriophage T4 and some observations concerning its middle promoters.噬菌体T4完整rIIB基因的克隆及其中间启动子的一些观察
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引用本文的文献

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Coordination of DNA ends during double-strand-break repair in bacteriophage T4.噬菌体T4双链断裂修复过程中DNA末端的协调
Genetics. 2002 Nov;162(3):1019-30. doi: 10.1093/genetics/162.3.1019.
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Mutations affecting translation of the bacteriophage T4 rIIB gene cloned in Escherichia coli.影响克隆于大肠杆菌中的噬菌体T4 rIIB基因翻译的突变。
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Wild-type bacteriophage T4 is restricted by the lambda rex genes.野生型噬菌体T4受λrex基因的限制。

本文引用的文献

1
rII cistrons of bacteriophage T4. DNA sequence around the intercistronic divide and positions of genetic landmarks.噬菌体T4的rII顺反子。顺反子间分隔区周围的DNA序列及遗传标记的位置。
J Mol Biol. 1981 Jul 5;149(3):337-76. doi: 10.1016/0022-2836(81)90477-0.
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Improved methods for maximizing expression of a cloned gene: a bacterium that synthesizes rabbit beta-globin.用于使克隆基因表达最大化的改进方法：一种合成兔β-珠蛋白的细菌。
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Plasmids containing many tandem copies of a synthetic lactose operator.
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Genetics. 1990 Jun;125(2):237-48. doi: 10.1093/genetics/125.2.237.
含有多个串联合成乳糖操纵子拷贝的质粒。
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In vivo expression of the rII region of bacteriophage T4 present in chimeric plasmids.
Mol Gen Genet. 1981;183(3):505-13. doi: 10.1007/BF00268772.
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Mutant of bacteriophage T4D affecting expression of many early genes.影响许多早期基因表达的噬菌体T4D突变体。
Nature. 1974 Jul 5;250(461):48-50. doi: 10.1038/250048a0.
6
Altered Cro repressors from engineered mutagenesis of a synthetic cro gene.通过对合成cro基因进行工程诱变得到的改变后的Cro阻遏蛋白。
Proc Natl Acad Sci U S A. 1985 Feb;82(4):1084-8. doi: 10.1073/pnas.82.4.1084.
7
A mutation that confers temperature sensitivity on the translation of rIIB in bacteriophage T4.
J Mol Biol. 1976 May 25;103(3):627-46. doi: 10.1016/0022-2836(76)90221-7.
8
Realignment of the genetic map of the terminus of the rIIB cistron of bacteriophage T4.噬菌体T4的rIIB顺反子末端遗传图谱的重新排列。
Genetics. 1979 Jul;92(3):711-20. doi: 10.1093/genetics/92.3.711.
9
Construction and properties of recombinant plasmids containing the rII genes of bacteriophage T4.含有噬菌体T4 rII基因的重组质粒的构建与特性
Mol Gen Genet. 1978 Feb 27;159(3):301-9. doi: 10.1007/BF00268267.
10
Intracellular DNA-protein complexes from bacteriophage T4-infected cells isolated by a rapid two-step procedure. Characterization and identification of the protein components.通过快速两步法从噬菌体T4感染细胞中分离出细胞内DNA-蛋白质复合物。蛋白质成分的表征与鉴定。
J Biol Chem. 1977 Apr 25;252(8):2734-41.