人小细胞肺癌细胞系的体外化学敏感性试验

Chemosensitivity test for human small cell lung cancer cell lines in vitro.

作者信息

Takahashi H, Saijo N, Shinkai T, Eguchi K, Sasaki Y, Tamura T, Sano T, Nakano H, Nakagawa K, Sakurai M

出版信息

Jpn J Clin Oncol. 1986 Sep;16(3):253-60.

PMID:3022034

Abstract

The in vitro response to seven chemotherapeutic drugs of three established human small cell lung cancer (SCLC) cell lines (NCI H69, H128, N231) was tested by a double soft agar clonogenic assay. Colony formation by the three cell lines was universally reduced more than 50% by continuous exposure to peak plasma concentrations of all the drugs. However by exposure to one-tenth of the peak plasma concentrations, the colony growth of H69 was reduced to 25.6% and 37.7% by etoposide and teniposide, respectively, and that of N231 was reduced to 46.7%, 39.0%, 27.5% by carboplatin, etoposide and teniposide, respectively. On the other hand colony formation by the three cell lines was not suppressed more than 50% by one-hour exposure to any of the drugs tested at one-tenth of the peak plasma concentrations. By one-hour exposure to drugs at the peak plasma concentrations, colony formation by H69, H128 and N231 was reduced more than 50% by cisplatin, etoposide, teniposide and nimustin, by adriamycin, teniposide and ACNU, and by adriamycin, etoposide, teniposide and nimustin, respectively. It was concluded that these three cell lines have similar sensitivity to seven drugs commonly used against small cell lung cancer.

摘要

通过双软琼脂克隆形成试验检测了三种已建立的人小细胞肺癌（SCLC）细胞系（NCI H69、H128、N231）对七种化疗药物的体外反应。连续暴露于所有药物的血浆峰值浓度时，这三种细胞系的集落形成普遍减少了50%以上。然而，暴露于血浆峰值浓度的十分之一时，依托泊苷和替尼泊苷分别使H69的集落生长减少至25.6%和37.7%，卡铂、依托泊苷和替尼泊苷分别使N231的集落生长减少至46.7%、39.0%、27.5%。另一方面，将三种细胞系暴露于血浆峰值浓度十分之一的任何一种受试药物1小时，其集落形成均未被抑制超过50%。将三种细胞系暴露于血浆峰值浓度的药物1小时，顺铂、依托泊苷、替尼泊苷和尼莫司汀使H69的集落形成减少超过50%，阿霉素、替尼泊苷和ACNU使H128的集落形成减少超过50%，阿霉素、依托泊苷、替尼泊苷和尼莫司汀使N231的集落形成减少超过50%。得出的结论是，这三种细胞系对常用于治疗小细胞肺癌的七种药物具有相似的敏感性。