体外干扰素产生：人白细胞在接触巨细胞病毒抗原后的诱导作用。
In vitro interferon production: induction in human leukocytes after exposure to cytomegalovirus antigens.
作者信息
Lun M T, Lorino G, Gaeta A, Dessy P, Cipriani P, Filadoro P, Capobianchi M R, Di Marco P, Mancini C
出版信息
Microbiologica. 1986 Oct;9(4):399-404.
In vitro lymphocytes from healthy donors with crude (HCMV), heat inactivated (HCMVi) and infected human fetal fibroblasts (HCMVFF) were stimulated. In all the experiments peak titers of alfa Interferon (IFN) were present 24 h after exposure to antigens. On the other hand, at least after seven days, only lymphocytes of seropositive donors stimulated by HCMVFF and HCMV showed gamma IFN production. Our HCMVFF gave much higher IFN yields than free virus. Our data suggest that HCMVFF and HCMV are recognised by lymphocytes of HCMV specific memory and HCMVFF give additional and more reproducible information on HCMV specific cellular immunity.
来自健康供体的体外淋巴细胞用粗制（HCMV）、热灭活（HCMVi）和感染人胎儿成纤维细胞（HCMVFF）进行刺激。在所有实验中，暴露于抗原后24小时出现α干扰素（IFN）的峰值滴度。另一方面，至少在七天后，只有受HCMVFF和HCMV刺激的血清阳性供体的淋巴细胞产生γ干扰素。我们的HCMVFF产生的IFN产量比游离病毒高得多。我们的数据表明，HCMVFF和HCMV被HCMV特异性记忆淋巴细胞识别，并且HCMVFF提供了关于HCMV特异性细胞免疫的更多且更可重复的信息。
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