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植物病原真菌中交替型NADH脱氢酶（NDH-2）的表达

Expression of alternative NADH dehydrogenases (NDH-2) in the phytopathogenic fungus .

作者信息

Matuz-Mares Deyamira, Matus-Ortega Genaro, Cárdenas-Monroy Christian, Romero-Aguilar Lucero, Villalobos-Rocha Juan Carlos, Vázquez-Meza Héctor, Guerra-Sánchez Guadalupe, Peña-Díaz Antonio, Pardo Juan Pablo

机构信息

Departamento de Bioquímica Facultad de Medicina Universidad Nacional Autónoma de México Ciudad de México México.

Departamento de Genética Molecular Instituto de Fisiología Celular Universidad Nacional Autónoma de México Ciudad de México México.

出版信息

FEBS Open Bio. 2018 Jul 5;8(8):1267-1279. doi: 10.1002/2211-5463.12475. eCollection 2018 Aug.

DOI:10.1002/2211-5463.12475
PMID:30221129
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6134880/
Abstract

Type 2 alternative NADH dehydrogenases (NDH-2) participate indirectly in the generation of the electrochemical proton gradient by transferring electrons from NADH and NADPH into the ubiquinone pool. Due to their structural simplicity, alternative NADH dehydrogenases have been proposed as useful tools for gene therapy of cells with defects in the respiratory complex I. In this work, we report the presence of three open reading frames, which correspond to NDH-2 genes in the genome of . These three genes were constitutively transcribed in cells cultured in YPD and minimal medium with glucose, ethanol, or lactate as carbon sources. Proteomic analysis showed that only two of the three NDH-2 were associated with isolated mitochondria in all culture media. Oxygen consumption by permeabilized cells using NADH or NADPH was different for each condition, opening the possibility of posttranslational regulation. We confirmed the presence of both external and internal NADH dehydrogenases, as well as an external NADPH dehydrogenase insensitive to calcium. Higher oxygen consumption rates were observed during the exponential growth phase, suggesting that the activity of NADH and NADPH dehydrogenases is coupled to the dynamics of cell growth.

摘要

2型替代NADH脱氢酶（NDH-2）通过将电子从NADH和NADPH转移到泛醌池中，间接参与电化学质子梯度的产生。由于其结构简单，替代NADH脱氢酶被认为是对呼吸复合体I有缺陷的细胞进行基因治疗的有用工具。在这项工作中，我们报告了三个开放阅读框的存在，它们对应于……基因组中的NDH-2基因。这三个基因在以葡萄糖、乙醇或乳酸作为碳源的YPD和基本培养基中培养的细胞中持续转录。蛋白质组学分析表明，在所有培养基中，三个NDH-2中只有两个与分离的线粒体相关。在每种条件下，使用NADH或NADPH的透化细胞的耗氧量不同，这为翻译后调控提供了可能性。我们证实了外部和内部NADH脱氢酶以及对钙不敏感的外部NADPH脱氢酶的存在。在指数生长期观察到更高的耗氧率，这表明NADH和NADPH脱氢酶的活性与细胞生长动态相关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53b3/6134880/22bb29c68a39/FEB4-8-1267-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53b3/6134880/a1247c67d8af/FEB4-8-1267-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53b3/6134880/8bcb438daba3/FEB4-8-1267-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53b3/6134880/f1651732951c/FEB4-8-1267-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53b3/6134880/87ad3b62c55f/FEB4-8-1267-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53b3/6134880/42a700ffffd6/FEB4-8-1267-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53b3/6134880/22bb29c68a39/FEB4-8-1267-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53b3/6134880/a1247c67d8af/FEB4-8-1267-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53b3/6134880/8bcb438daba3/FEB4-8-1267-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53b3/6134880/f1651732951c/FEB4-8-1267-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53b3/6134880/87ad3b62c55f/FEB4-8-1267-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53b3/6134880/42a700ffffd6/FEB4-8-1267-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53b3/6134880/22bb29c68a39/FEB4-8-1267-g006.jpg

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