1 Department of Chemical Engineering, Faculty of Engineering, Kyushu University , Fukuoka, Japan .
2 Graduate School of Systems Life Sciences, Kyushu University , Fukuoka, Japan .
Tissue Eng Part A. 2019 Apr;25(7-8):563-574. doi: 10.1089/ten.TEA.2018.0165. Epub 2018 Oct 27.
In this study, we fabricated innervated skeletal muscle tissue constructs comprising C2C12 myoblasts and PC12 neural cells using a magnetic force-based tissue engineering technique. We found that the C2C12/PC12 co-culture enhanced neural differentiation of PC12 cells and sarcomere formation of C2C12 myotubes, accompanying with neuromuscular junction formation. The innervated skeletal muscle tissue constructs generated significantly higher contractile forces compared with aneural (C2C12 monoculture) skeletal muscle tissue constructs. These innervated skeletal muscle tissue constructs can be a useful tool for drug testing and biological research for neuromuscular diseases.
在这项研究中，我们使用基于磁力的组织工程技术构建了包含 C2C12 成肌细胞和 PC12 神经细胞的神经支配骨骼肌组织构建体。我们发现，C2C12/PC12 共培养增强了 PC12 细胞的神经分化和 C2C12 肌管的肌节形成，伴随着神经肌肉接头的形成。与无神经（C2C12 单细胞培养）骨骼肌组织构建体相比，这些神经支配的骨骼肌组织构建体产生的收缩力显著更高。这些神经支配的骨骼肌组织构建体可以成为神经肌肉疾病药物测试和生物学研究的有用工具。
