伐昔洛韦对患有马多结节性肺纤维化的马匹中马疱疹病毒5型病毒动力学的影响。

Effect of valacyclovir on EHV-5 viral kinetics in horses with equine multinodular pulmonary fibrosis.

作者信息

Easton-Jones Charlotte A, Madigan John E, Barnum Samantha, Maxwell Lara K, Taylor Sandra D, Arnesen Terry, Pusterla Nicola

机构信息

Willian R Pritchard Veterinary Medical Teaching Hospital, University of California Davis, California.

Department of Medicine and Epidemiology, University of California, Davis, California.

出版信息

J Vet Intern Med. 2018 Sep;32(5):1763-1767. doi: 10.1111/jvim.15230. Epub 2018 Sep 17.

DOI:10.1111/jvim.15230

PMID:30221792

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6189341/

Abstract

BACKGROUND

Equine herpesvirus-5 is commonly isolated from the lungs of horses with EMPF, suggesting an etiological link. Valacyclovir is used empirically to treat EMPF; however, no data is available concerning its impact on EHV-5 viral kinetics.

OBJECTIVES

To determine the effect of oral administration of valacyclovir on EHV-5 viral load measured by qPCR in blood, nasal secretions (NS) and BALF in horses with EMPF.

ANIMALS

Six horses diagnosed with EMPF.

METHODS

A prospective clinical trial was performed. Horses received 10 days of PO administered valacyclovir (loading dose 30 mg/kg, maintenance dose 20 mg/kg). Blood, NS, and BALF were collected for EHV-5 viral kinetics analyses during treatment. Blood and NS were collected every other day. BALF was collected on day 0 and day 10.

RESULTS

There was no statistical difference in median EHV-5 viral load between day 0 and day 10 for all samples tested. In blood median EHV-5 viral load was 7676 (range 575-39 781) on day 0 and 6822 (range 1136-18 635) glycoprotein B (gB) gene copies per million cells on day 10. For NS median EHV-5 viral load was 2.944 × 10 (range 184 691-3.394 × 10 ) on day 0 and 8.803 × 10 (range 251 186-9.868 × 10 ) gB gene copies per million cells on day 10. For BALF median EHV-5 viral load was 59,842 (range 61-315 655) on day 0 and 185 083 (range 3562-542 417) gB gene copies per million cells on day 10.

CONCLUSIONS AND CLINICAL IMPORTANCE

Valacyclovir might not be an effective short-term antiviral treatment but efficacy in treatment of EMPF is unknown.

摘要

背景

马疱疹病毒5型通常从患有马流行性间质性肺炎（EMPF）的马的肺部分离得到，提示存在病因学关联。伐昔洛韦被经验性地用于治疗EMPF；然而，尚无关于其对EHV-5病毒动力学影响的数据。

目的

确定口服伐昔洛韦对通过qPCR测定的患有EMPF的马的血液、鼻分泌物（NS）和支气管肺泡灌洗液（BALF）中EHV-5病毒载量的影响。

动物

6匹被诊断为EMPF的马。

方法

进行了一项前瞻性临床试验。马接受为期10天的口服伐昔洛韦治疗（负荷剂量30mg/kg，维持剂量20mg/kg）。在治疗期间采集血液、NS和BALF用于EHV-5病毒动力学分析。每隔一天采集血液和NS。在第0天和第10天采集BALF。

结果

对于所有测试样本，第0天和第10天之间EHV-5病毒载量中位数无统计学差异。血液中EHV-5病毒载量中位数在第0天为7676（范围575 - 39781）每百万细胞中糖蛋白B（gB）基因拷贝数，在第10天为6822（范围1136 - 18635）。对于NS，EHV-5病毒载量中位数在第0天为2.944×10（范围184691 - 3.394×10）每百万细胞中gB基因拷贝数，在第10天为8.803×10（范围251186 - 9.868×10）。对于BALF，EHV-5病毒载量中位数在第0天为59842（范围61 - 315655）每百万细胞中gB基因拷贝数，在第10天为185083（范围3562 - 542417）。