Domoslawska Anna, Zdunczyk Slawomir, Franczyk Monika, Kankofer Marta, Janowski Tomasz
Department of Animal Reproduction with Clinic, University of Warmia and Mazury, Olsztyn, Poland.
Department of Biochemistry, Faculty of Veterinary Medicine, University of Life Sciences, Lublin, Poland.
Reprod Domest Anim. 2019 Feb;54(2):252-257. doi: 10.1111/rda.13345. Epub 2018 Oct 10.
The aim of this study was to evaluate the total antioxidant capacity and protein peroxidation intensity in seminal plasma of infertile and fertile dogs. The study was conducted on 10 infertile and 10 fertile dogs of various breeds. Infertility was defined as conception failure at least three matings with different bitches. Semen was collected by manual manipulation. The sperm concentration and motility parameters were evaluated using CASA Hamilton Thorne, Vers. IVOS 12.3. The morphology of spermatozoa and the percentage of live and dead sperm cells were assessed microscopically, total antioxidant capacity and the content of SH-groups in seminal plasma were determined spectrophotometrically, the contents of protein peroxidation markers in seminal plasma, bityrosine and formylokinurenine, were determined using spectrofluorimetric methods. Sperm concentration and total sperm count were significantly (p < 0.05) lower in infertile dogs than in fertile dogs (99.92 ± 3 0.05 × 10 /ml vs. 282.07 ± 48.27 × 10 /ml; 214.19 ± 114.74 × 10 vs. 747.57 ± 210.94 × 10 , respectively). The percentage of spermatozoa with normal morphology and the most determined motility parameters differed significantly (p < 0.05) between both groups. The mean values of total antioxidant capacity in the seminal plasma were significantly (p < 0.05) lower (19.95 ± 20.94 vs. 25.66 ± 23.18 µmol/g protein), whereas the mean contents of bityrosine and formylokinurenine in seminal plasma were significantly (p < 0.05) higher in infertile dogs than in fertile dogs (3.71 ± 4.83 µg/mg protein vs. 1.55 ± 2.00 µg/mg protein and 0.37 ± 0.45 µg/mg protein vs. 0.14 ± 0.08 µg/mg protein, respectively). In conclusion, the obtained results suggest that the poor semen quality and infertility in dogs could be associated with lowered total antioxidant capacity and increased protein peroxidation in seminal plasma as a consequence of oxidative stress.
本研究的目的是评估不育和可育犬精液中总抗氧化能力及蛋白质过氧化强度。该研究选取了10只不育犬和10只不同品种的可育犬。不育定义为与不同母犬至少交配三次仍受孕失败。通过人工采精获取精液。使用CASA Hamilton Thorne Vers. IVOS 12.3评估精子浓度和活力参数。通过显微镜评估精子形态以及活精子和死精子细胞的百分比，用分光光度法测定精液中总抗氧化能力和SH基团含量，用荧光分光光度法测定精液中蛋白质过氧化标志物双酪氨酸和甲酰犬尿氨酸的含量。不育犬的精子浓度和总精子数显著低于可育犬（分别为99.92±30.05×10⁶/ml对282.07±48.27×10⁶/ml；214.19±114.74×10⁶对747.57±210.94×10⁶）（p<0.05）。两组间具有正常形态的精子百分比及大多数测定的活力参数存在显著差异（p<0.05）。精液中总抗氧化能力的平均值显著较低（19.95±20.94对25.66±23.18μmol/g蛋白质）（p<0.05），而不育犬精液中双酪氨酸和甲酰犬尿氨酸的平均含量显著高于可育犬（分别为3.71±4.83μg/mg蛋白质对1.55±2.00μg/mg蛋白质和0.37±0.45μg/mg蛋白质对0.14±0.08μg/mg蛋白质）（p<0.05）。总之，所得结果表明犬精液质量差和不育可能与氧化应激导致的精液中总抗氧化能力降低及蛋白质过氧化增加有关。
