Dorostghoal M, Kazeminejad S R, Shahbazian N, Pourmehdi M, Jabbari A
Toxicology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Department of Genetics, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Andrologia. 2017 Dec;49(10). doi: 10.1111/and.12762. Epub 2017 Jan 26.
Evidence suggests that disturbing the balance between reactive oxygen species levels and antioxidant contents in seminal plasma leads to oxidative stress resulting in male infertility. This study was carried out to identifying clinical significance of seminal oxidative stress and sperm DNA fragmentation in treatment strategies of male infertility in southwest Iran. Sperm parameters, lipid peroxidation and activity of antioxidant enzymes were assessed in fertile (n = 105) and infertile (n = 112) men. Malondialdehyde (MDA) levels in seminal plasma were found to be higher significantly (p < .001) in patients. Superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities in seminal plasma were significantly (p < .001) lower in infertile men. Significant negative correlations were observed between MDA levels and sperm motility and normal morphology. Spermatozoa with fragmented DNA were higher (p < .001) in infertile men and significantly correlated with MDA levels and SOD and GPx activities. MDA of 4.2 nmol/ml, SOD of 4.89 U/ml and GPx of 329.6 mU/ml were optimum cut-off limits to discriminate infertile patients from fertile men. The results show the leading role of oxidative stress in aetiology of male infertility in southwest Iran and indicate that evaluation of seminal antioxidant status and DNA integrity can be helpful in men attending infertility clinics during fertility assessment.
有证据表明，精液中活性氧水平与抗氧化物质含量之间的平衡被打破会导致氧化应激，进而引发男性不育。本研究旨在确定精液氧化应激和精子DNA碎片化在伊朗西南部男性不育治疗策略中的临床意义。对105名有生育能力的男性和112名不育男性的精子参数、脂质过氧化和抗氧化酶活性进行了评估。结果发现，不育患者精液中丙二醛（MDA）水平显著更高（p <.001）。不育男性精液中超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）活性显著更低（p <.001）。MDA水平与精子活力和正常形态之间存在显著负相关。不育男性中DNA碎片化的精子比例更高（p <.001），且与MDA水平以及SOD和GPx活性显著相关。4.2 nmol/ml的MDA、4.89 U/ml的SOD和329.6 mU/ml的GPx是区分不育患者和有生育能力男性的最佳临界值。结果表明氧化应激在伊朗西南部男性不育病因中起主导作用，并表明在生育评估期间，对精液抗氧化状态和DNA完整性的评估对不育门诊男性可能有帮助。
