Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Kingdom of Saudi Arabia. E-mail.
Saudi Med J. 2020 Mar;41(3):238-246. doi: 10.15537/smj.2020.3.24975.
To assess the effect of seminal redox status on lipid peroxidation (LPO), apoptosis and integrity of sperm DNA in infertile males. Methods: In this case-control study, the total antioxidant status (TAS) and reactive oxygen species (ROS) levels were analyzed within the seminal plasma of fertile normozoospermic, n=40 and infertile (asthenozoospermic, n=30; oligoasthenoteratozoospermic, n=30) males. Additionally, the level of 4-hydroxynonenal (4-HNE), DNA fragmentation, and caspase-3 activity were estimated in the spermatozoa.
Significantly (p less than 0.001) increased seminal ROS level with decreased TAS scores was observed in the infertile groups compared to normozoospermics. The infertile males showed marked elevated (p less than 0.001) levels of 4-HNE, DNA fragmentation and caspase-3 activity compared to normozoospermics, which was positively correlated to increased seminal ROS levels and negatively to the TAS score in the studied groups. Seminal ROS level was significantly inverse correlated to the semen parameters. Additionally, a strong negative correlation between DNA fragmentation, LPO, caspase-3activity and seminal parameters were observed. Conclusion: Seminal oxidative stress is a potential risk factor for LPO, DNA damage, and apoptosis in spermatozoa, which can affect semen quality and male fertility. Thus, in addition to conventional seminological parameters, measurement of seminal oxidative stress and sperm DNA integrity may also be employed to investigate the functional integrity of spermatozoa at the molecular level.
评估精液氧化还原状态对精子脂质过氧化 (LPO)、凋亡和 DNA 完整性的影响。
在这项病例对照研究中，分析了 40 名正常生育力、正常形态精子的男性（对照组）和 30 名弱精症（精子活力低下）、30 名少弱畸形精子症（精子数量少且形态异常）男性（病例组）精液中的总抗氧化状态 (TAS) 和活性氧 (ROS) 水平。此外，还评估了精子中的 4-羟基壬烯醛 (4-HNE)、DNA 碎片化和半胱氨酸天冬氨酸蛋白酶-3 (caspase-3) 活性。
与对照组相比，病例组的精液 ROS 水平显著升高（p 小于 0.001），而 TAS 评分则降低。与对照组相比，病例组的 4-HNE、DNA 碎片化和 caspase-3 活性水平明显升高（p 小于 0.001），这些标志物与研究组中增加的 ROS 水平呈正相关，与 TAS 评分呈负相关。ROS 水平与精液参数呈显著负相关。此外，还观察到 DNA 碎片化、LPO、caspase-3 活性与精液参数之间存在很强的负相关。
精液氧化应激是精子 LPO、DNA 损伤和凋亡的潜在危险因素，可能影响精液质量和男性生育能力。因此，除了常规的精液参数外，还可以测量精液氧化应激和精子 DNA 完整性，以从分子水平研究精子的功能完整性。
