Busija D W, Leffler C W, Beasley D G
Pediatr Res. 1986 Oct;20(10):973-6. doi: 10.1203/00006450-198610000-00016.
We examined effects of topical application of leukotrienes (LT) C4, D4, and E4 on cerebral arteries of newborn pigs in vivo. Diameters of pial arteries were measured using a cranial window method during application of artificial cerebrospinal fluid without drug, and cerebrospinal fluid containing LT C4, D4, and E4 (1, 10, 100, 1000, and 5000 ng/ml). Control diameters ranged from 51-345 micron. All three LT constricted pial arteries in a dose-dependent manner, with a threshold for detectable response at 10 ng/ml (7 +/- 3% for LTD4). The magnitude of constrictor response at the highest dose was 23 +/- 3% for LTC4, 17 +/- 2% for LTD4, and 17 +/- 3% for LTE4. The specific receptor antagonist FPL 55712 blocked the constrictor response to LT. We conclude that LT are potent constrictors of cerebral arteries in newborn pigs.
我们在新生猪体内研究了局部应用白三烯（LT）C4、D4和E4对脑动脉的影响。在应用不含药物的人工脑脊液以及含有LT C4、D4和E4（1、10、100、1000和5000 ng/ml）的脑脊液期间，使用颅窗法测量软脑膜动脉的直径。对照直径范围为51 - 345微米。所有三种LT均以剂量依赖性方式收缩软脑膜动脉，可检测到反应的阈值为10 ng/ml（LTD4为7±3%）。最高剂量时收缩反应的幅度，LTC4为23±3%，LTD4为17±2%，LTE4为17±3%。特异性受体拮抗剂FPL 55712可阻断对LT的收缩反应。我们得出结论，LT是新生猪脑动脉的强效收缩剂。
