Busija D W, Leffler C W
Prostaglandins. 1986 Dec;32(6):803-11. doi: 10.1016/0090-6980(86)90091-2.
We investigated effects of exogenous leukotrienes (C4, D4, or E4) on levels of prostanoids in cerebrospinal fluid in newborn pigs (1-5 days). A "closed" cranial window was placed over the parietal cortex. Pial arterial diameter was measured with a microscope and electronic micrometer system. Levels in cerebrospinal fluid (CSF) of 6-keto-Prostaglandin F1 alpha (6-keto-PGF1 alpha), Thromboxane B2 (TXB2), and Prostaglandin E2 (PGE2) were measured by radioimmunoassay. Topical application of leukotrienes C4, D4, or E4 (5,000 ng/ml) similarly constricted pial arteries by 15 +/- 2% (n = 14) (mean +/- SEM). In addition, leukotrienes increased levels of 6-keto-PGF1 alpha from 806 +/- 136 to 1,612 +/- 304 pg/ml (n = 13), TXB2 from 161 +/- 31 to 392 +/- 81 pg/ml (n = 10), and PGE2 from 2,271 +/- 342 to 4,636 +/- 740 pg/ml (n = 13). Each type of leukotriene had similar effects on prostanoid synthesis. In other experiments (n = 5), we found that 2.0 ng/ml PGE2 in CSF dilated pial arteries by 24 +/- 8% and that 1.0 ng/ml PGI2 dilated pial arteries by 15 +/- 6%. These results indicate that leukotrienes are able to increase levels of prostanoids in cerebral cortex.
我们研究了外源性白三烯（C4、D4或E4）对新生猪（1 - 5日龄）脑脊液中前列腺素水平的影响。在顶叶皮质上方放置一个“封闭”的颅骨视窗。用显微镜和电子测微系统测量软脑膜动脉直径。通过放射免疫分析法测量脑脊液（CSF）中6 - 酮 - 前列腺素F1α（6 - 酮 - PGF1α）、血栓素B2（TXB2）和前列腺素E2（PGE2）的水平。局部应用白三烯C4、D4或E4（5000 ng/ml）同样使软脑膜动脉收缩15±2%（n = 14）（平均值±标准误）。此外，白三烯使6 - 酮 - PGF1α水平从806±136 pg/ml升高至1612±304 pg/ml（n = 13），TXB2从161±31 pg/ml升高至392±81 pg/ml（n = 10），PGE2从2271±342 pg/ml升高至4636±740 pg/ml（n = 13）。每种白三烯对前列腺素合成的影响相似。在其他实验（n = 5）中，我们发现脑脊液中2.0 ng/ml的PGE2使软脑膜动脉扩张24±8%，1.0 ng/ml的前列环素（PGI2）使软脑膜动脉扩张15±6%。这些结果表明白三烯能够增加大脑皮质中前列腺素的水平。
