Shimomura H, Suzuki N, Garbers D L
Peptides. 1986 May-Jun;7(3):491-5. doi: 10.1016/0196-9781(86)90019-7.
Two peptides associated with the eggs of the sea urchin, Lytechinus pictus, which stimulate L. pictus but not Arbacia punctulata sperm respiration rates, were purified and their amino acid sequences determined. The peptides (Gly-Phe-Asp-Leu-Thr-Gly-Gly-Gly-Val-Gln and Phe-Asp-Leu-Thr-Gly-Gly-Gly-Val-Gln) were found to be structurally similar to the peptide, speract (Gly-Phe-Asp-Leu-Asn-Gly-Gly-Gly-Val-Gly). Chemical synthesis of the two peptides confirmed their ability to activate sperm respiration. The peptides had equivalent biological activity with half-maximal stimulation of respiration rates and of cyclic nucleotide concentrations occurring at 60 pM and 700 pM, respectively. Addition of the peptides to intact spermatozoa resulted in the rapid appearance of a newly-stained protein on Na X dodecyl X SOl polyacrylamide gels (Mr = 140,000); one-half maximal formation of the Mr 140,000 protein occurred at about 20-100 nM peptide.
两种与海胆（Lytechinus pictus）卵相关的肽被纯化，它们能刺激Lytechinus pictus精子的呼吸速率，但不刺激Arbacia punctulata精子的呼吸速率，并测定了它们的氨基酸序列。发现这些肽（甘氨酸-苯丙氨酸-天冬氨酸-亮氨酸-苏氨酸-甘氨酸-甘氨酸-甘氨酸-缬氨酸-谷氨酰胺和苯丙氨酸-天冬氨酸-亮氨酸-苏氨酸-甘氨酸-甘氨酸-甘氨酸-缬氨酸-谷氨酰胺）在结构上与肽speract（甘氨酸-苯丙氨酸-天冬氨酸-亮氨酸-天冬酰胺-甘氨酸-甘氨酸-甘氨酸-缬氨酸-甘氨酸）相似。这两种肽的化学合成证实了它们激活精子呼吸的能力。这些肽具有同等的生物活性，分别在60 pM和700 pM时对呼吸速率和环核苷酸浓度产生半数最大刺激。将这些肽添加到完整的精子中，在十二烷基硫酸钠聚丙烯酰胺凝胶上迅速出现一种新染色的蛋白质（分子量 = 140,000）；在约20 - 100 nM肽时，分子量为140,000的蛋白质形成达到半数最大量。
