来自粗刻疣海胆的一组新型精子激活肽。

A novel group of sperm activating peptides from the sea urchin Glyptocidaris crenularis.

Suzuki N, Yoshino K, Kurita M, Nomura K, Yamaguchi M

Noto Marine Laboratory, Kanazawa University, Ishikawa, Japan.

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1988;90(2):305-11. doi: 10.1016/0742-8413(88)90003-5.

Abstract

Six new sperm activating peptides were purified from the egg jelly of the sea urchin Glyptocidaris crenularis and their amino acid sequences were determined as follows: Ser-Ala-Lys-Leu-Cys-Pro-Gly-Gly-Asn-Cys-Val, Lys-Leu-Cys-Pro-Gly-Gly-Asn-Cys-Val, Leu-Cys-Pro-Gly-Gly-Asn-Cys-Val, Ser-Phe-Lys-Leu-Cys-Pro-Gly-Gly-Gln-Cys-Val, Lys-Leu-Cys-Pro-Gly-Gly-Gln-Cys-Val and Leu-Cys-Pro-Gly-Gly-Gln-Cys-Val. 2. The peptides were specific for G. crenularis spermatozoa and caused significant increases of sperm respiration rates and sperm cyclic nucleotide concentrations at concentrations as low as 10(-10) M. 3. The addition of the peptides to intact spermatozoa resulted in the change of the apparent mol. wt of a sperm protein from 195,000 to 200,000.

摘要

从刻肋海胆（Glyptocidaris crenularis）的卵胶中纯化出六种新的精子激活肽，其氨基酸序列测定如下：Ser-Ala-Lys-Leu-Cys-Pro-Gly-Gly-Asn-Cys-Val、Lys-Leu-Cys-Pro-Gly-Gly-Asn-Cys-Val、Leu-Cys-Pro-Gly-Gly-Asn-Cys-Val、Ser-Phe-Lys-Leu-Cys-Pro-Gly-Gly-Gln-Cys-Val、Lys-Leu-Cys-Pro-Gly-Gly-Gln-Cys-Val和Leu-Cys-Pro-Gly-Gly-Gln-Cys-Val。2. 这些肽对刻肋海胆精子具有特异性，在低至10^(-10) M的浓度下就能显著提高精子呼吸速率和精子环核苷酸浓度。3. 将这些肽添加到完整精子中会导致一种精子蛋白的表观分子量从195,000变为200,000。