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在实验室条件下,短小芽孢杆菌对丽蝇幼虫和成虫(双翅目:丽蝇科)的生物活性。

Bioactivity under laboratory conditions of Brevibacillus laterosporus towards larvae and adults of Chrysomya putoria (Diptera: Calliphoridae).

机构信息

Laboratório de Entomologia Médica e Forense, Pavilhão Herman Lent, Sala 14, Instituto Oswaldo Cruz/ Fiocruz. Av. Brasil, 4365, Rio de Janeiro CEP 21040-360, Brazil.

Laboratório de Biologia Computacional e Sistemas, Pavilhão Leônidas Deane, sala 301, Instituto Oswaldo Cruz/Fiocruz. Av. Brasil, 4365, Rio de Janeiro CEP 21045-900, Brazil.

出版信息

J Invertebr Pathol. 2018 Oct;158:52-54. doi: 10.1016/j.jip.2018.09.003. Epub 2018 Sep 14.

DOI:10.1016/j.jip.2018.09.003
PMID:30222956
Abstract

Brevibacillus laterosporus was tested for entomopathogenic activity towards larvae and adults of Chrysomya putoria (Diptera: Calliphoridae) under laboratory conditions. Sublethal effects related to feeding activity or development were observed, including reduction in larval weight gain, probably by inhibition of feeding, and variation in the duration of the developmental stages of the insect. Larval mortality was dose dependent following ingestion. The experiments were performed with newly emerged adults exposed to a sugar based diet containing spore suspensions. Concentrations of 1.13 × 10 CFU/ml caused 70.5% of mortality. The present study highlights the potential of B. laterosporus to control populations of C. putoria, a dipteran of medical-veterinary and sanitary importance, both in larval and adult stages.

摘要

在实验室条件下,测试了短芽孢杆菌对丽蝇(双翅目:丽蝇科)幼虫和成虫的昆虫病原活性。观察到与摄食活动或发育有关的亚致死效应,包括幼虫体重增加减少,可能是由于摄食抑制,以及昆虫发育阶段的持续时间变化。幼虫在摄入后死亡率与剂量有关。实验是用新羽化的成虫暴露在含有孢子悬浮液的基于糖的饮食中进行的。浓度为 1.13×10 CFU/ml 时,死亡率达到 70.5%。本研究强调了短芽孢杆菌控制具有医学兽医和卫生重要性的双翅目蝇属种群的潜力,无论是在幼虫还是成虫阶段。

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