International Center for Ecology, Meteorology, and Environment, School of Applied Meteorology, Nanjing University of Information Science & Technology, Nanjing 210044, China.
International Center for Ecology, Meteorology, and Environment, School of Applied Meteorology, Nanjing University of Information Science & Technology, Nanjing 210044, China.
Ecotoxicol Environ Saf. 2018 Dec 15;165:505-509. doi: 10.1016/j.ecoenv.2018.09.034. Epub 2018 Sep 14.
Atmospheric fine particulate matters (PM) induce adverse human health effects through inhalation, and the harmful effects of PM are determined not only by its air concentrations, but also by the particle components varied temporally. To investigate seasonal differences of the aerosol toxicity effects including cell viability and membrane damage, cell oxidative stress and responses of inflammatory cytokines, the human lung epithelial cells (A549) were exposed to PM samples collected in both summer and winter by the in vitro toxicity bioassays. Toxicological results showed that, the PM led to the cell viability decrease, cell membrane injury, oxidative stress level increase and inflammatory responses in a dose-dependent manner. Temporally, the cytotoxicity of winter PM was higher than summer of this studied industrial area of Nanjing, China. According to the different contents of heavy metals accumulated in PM, the transition metals such as Cu might be an important contributor to the aerosol cell toxicity.
大气细颗粒物（PM）通过吸入对人体健康产生不良影响，其危害不仅取决于其空气浓度，还取决于随时间变化的颗粒成分。为了研究气溶胶毒性作用的季节性差异，包括细胞活力和膜损伤、细胞氧化应激和炎症细胞因子的反应，我们采用体外毒性生物测定法，用采集自夏季和冬季的 PM 样本对人肺上皮细胞（A549）进行了暴露实验。毒理学结果表明，PM 会导致细胞活力下降、细胞膜损伤、氧化应激水平升高和炎症反应，且呈剂量依赖性。就时间而言，来自中国南京这一工业区的冬季 PM 的细胞毒性高于夏季。根据 PM 中积累的重金属含量不同，Cu 等过渡金属可能是气溶胶细胞毒性的一个重要贡献者。
