School of Life Sciences, Tsinghua University, Beijing, China.
State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences-Beijing (PHOENIX Center), Beijing Institute of Lifeomics, China.
FEBS Lett. 2018 Nov;592(22):3670-3682. doi: 10.1002/1873-3468.13255. Epub 2018 Nov 2.
Embryonic stem cells (ESCs) are characterized by a dual capacity, self-renewal and pluripotency, which can be regulated by metabolism. A better understanding of ESC metabolism and regulatory mechanisms is pivotal for research into development, ageing, and cancer treatment. However, a systematic and comprehensive delineation of human ESC metabolism is still lacking. Here, we reconstructed the first genome-scale metabolic model (GEM) of human ESCs (hESCs). By GEM simulation and analyses, hESC global metabolic characteristics including essential metabolites and network motifs were identified. Potential metabolic subsystems responsible for self-renewal and pluripotency were also identified by analyses and experiments. This first GEM of hESCs provides a novel view and resource for stem cell metabolism research and will contribute to the elucidation of their metabolic characteristics.
胚胎干细胞（ESCs）的特征是具有自我更新和多能性的双重能力，这可以通过代谢来调节。更好地了解 ESC 代谢和调节机制对于研究发育、衰老和癌症治疗至关重要。然而，人类 ESC 代谢的系统和全面描述仍然缺乏。在这里，我们构建了人类胚胎干细胞（hESCs）的第一个基因组规模代谢模型（GEM）。通过 GEM 模拟和分析，确定了 hESC 的全局代谢特征，包括必需代谢物和网络基元。通过分析和实验，还确定了负责自我更新和多能性的潜在代谢子系统。hESCs 的这个第一个 GEM 为干细胞代谢研究提供了新的视角和资源，并将有助于阐明它们的代谢特征。
