Cancer Biomark. 2018;23(3):381-389. doi: 10.3233/CBM-181413.
Ubiquitin-specific protease 22 (USP22), as one of the 11 death-from-cancer signature genes, presented high expression in a variety of tumors. Previous studies showed that USP22 played a significant role in cell-cycle, oncogenesis, clinicopathology and survival. Our studies have presented USP22 was over-expressed in glioma tissue and the patients with high expression of USP22 had a poor survival than that with low expression of USP22. However, the concrete effect of USP22 on biological behavior in glioma cells has been rarely reported. The study aimed to clear the effect of USP22 on cell proliferation, migration and invasion in glioma. Using siRNA, USP22 was knocked down in U251 and U87 glioma cells and successful transfection effect was validated. Cell proliferation, migration and invasion were observed by the methods of EdU, Wound healing and Transwell assay, separately. At the same time, the expression of MMP2 was detected by Gelatin zymography after transfecting siRNAs. After the knockdown of USP22 by siRNA, the abilities of glioma cell proliferation, migration and invasion were decreased, accompanying, the expression of MMP2 was also decreased. We drew a conclusion that USP22 could increase the abilities of proliferation, migration and invasion of glioma cells, and promote the growth and development of glioma.
泛素特异性蛋白酶 22（USP22）作为 11 个癌症相关死亡基因签名之一，在多种肿瘤中呈现高表达。先前的研究表明，USP22 在细胞周期、致癌作用、临床病理和生存中发挥着重要作用。我们的研究表明，USP22 在胶质瘤组织中过表达，并且 USP22 高表达的患者的生存时间比 USP22 低表达的患者差。然而，USP22 对胶质瘤细胞生物学行为的具体影响很少有报道。本研究旨在阐明 USP22 对胶质瘤细胞增殖、迁移和侵袭的影响。通过使用 siRNA 敲低 U251 和 U87 胶质瘤细胞中的 USP22，并验证了成功的转染效果。分别通过 EdU、划痕愈合和 Transwell 测定法观察细胞增殖、迁移和侵袭。同时，在转染 siRNA 后通过明胶酶谱法检测 MMP2 的表达。通过 siRNA 敲低 USP22 后，胶质瘤细胞的增殖、迁移和侵袭能力降低，同时 MMP2 的表达也降低。我们得出结论，USP22 可以增加胶质瘤细胞的增殖、迁移和侵袭能力，促进胶质瘤的生长和发展。
