1 Pontificia Universidade Catolica do Parana, Curitiba, Paraná, Brazil.
2 Colorado State University, Fort Collins, Colorado, USA.
J Biomater Appl. 2018 Sep;33(3):410-421. doi: 10.1177/0885328218797549.
In this study, we investigate the formation of calcium and phosphorus-doped TiO nanotubes, produced by potentiostatic anodization of Ti in viscous electrolyte-containing HF and Ca/P ions. Characterization of the produced oxide layer was conducted using scanning electron microscopy, glancing-angle X-ray diffraction, X-ray photoelectron spectroscopy, contact angle, and protein adsorption measurements. Adipose-derived stem cells were used to study material cytotoxicity, cell viability and proliferation, and cell morphology and growth. To evaluate the adipose-derived stem-cell differentiation, we investigated the expression of osteocalcin and osteopontin by cells as well as calcium mineralization. Results show that it was possible to produce a superhydrophilic titanium oxide nanotube layer with incorporation of Ca and P ions. The presence of Ca and P in the oxide layer not only improved the cell adhesion and proliferation but also stimulated the production of key marker proteins indicating differentiation of cells.
在这项研究中，我们通过在含有 HF 和 Ca/P 离子的粘性电解质中对 Ti 进行恒电位阳极氧化，研究了钙和磷掺杂的 TiO 纳米管的形成。使用扫描电子显微镜、掠角 X 射线衍射、X 射线光电子能谱、接触角和蛋白质吸附测量对所制备的氧化层进行了表征。使用脂肪来源的干细胞来研究材料的细胞毒性、细胞活力和增殖以及细胞形态和生长。为了评估脂肪来源的干细胞分化，我们研究了细胞表达的骨钙素和骨桥蛋白以及钙的矿化。结果表明，有可能在掺入 Ca 和 P 离子的情况下制备超亲水氧化钛纳米管层。氧化层中 Ca 和 P 的存在不仅改善了细胞的黏附和增殖，而且还刺激了关键标记蛋白的产生，表明细胞的分化。
