School of Advanced Materials Discovery, Colorado State University, Department of Bioengineering, George Mason University, Fort Collins, CO, USA, Fairfax, VA, USA.
Department of Mechanical Engineering, Pontifícia Universidade Católica do Paraná, PR, Brazil.
Nanoscale. 2024 Jul 4;16(26):12510-12522. doi: 10.1039/d4nr01123f.
Titanium-based orthopedic implants are gaining popularity in recent years due to their excellent biocompatibility, superior corrosion resistance and lightweight properties. However, these implants often fail to perform effectively due to poor osseointegration. Nanosurface modification approaches may help to resolve this problem. In this work, TiO nanotube (NT) arrays were fabricated on commercially available pure titanium (Ti) surfaces by anodization and annealing. Then, zinc (Zn) and strontium (Sr), important for cell signaling, were doped on the NT surface by hydrothermal treatment. This very simple method of Zn and Sr doping takes less time and energy compared to other complicated techniques. Different surface characterization tools such as scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), energy-dispersive X-ray spectroscopy (EDS), static water contact angle, X-ray diffraction (XRD) and nanoindentation techniques were used to evaluate the modified surfaces. Then, adipose derived stem cells (ADSCs) were cultured with the surfaces to evaluate cell adhesion, proliferation, and growth on the surfaces. After that, the cells were differentiated towards osteogenic lineage to evaluate alkaline phosphatase (ALP) activity, osteocalcin expression, and calcium phosphate mineralization. Results indicate that NT surfaces doped with Zn and Sr had significantly enhanced ADSC adhesion, proliferation, growth, and osteogenic differentiation compared to an unmodified surface, thus confirming the enhanced performance of these surfaces.
近年来，基于钛的骨科植入物因其出色的生物相容性、优异的耐腐蚀性和轻巧的特性而受到关注。然而，由于骨整合不良，这些植入物往往无法有效发挥作用。纳米表面改性方法可能有助于解决这个问题。在这项工作中，通过阳极氧化和退火在市售纯钛（Ti）表面上制造了 TiO 纳米管（NT）阵列。然后，通过水热处理在 NT 表面掺杂锌（Zn）和锶（Sr），这对细胞信号很重要。与其他复杂技术相比，这种 Zn 和 Sr 掺杂的非常简单的方法需要更少的时间和能量。使用不同的表面特性表征工具，如扫描电子显微镜（SEM）、X 射线光电子能谱（XPS）、能谱（EDS）、静态水接触角、X 射线衍射（XRD）和纳米压痕技术来评估改性表面。然后，将脂肪来源的干细胞（ADSCs）与表面一起培养，以评估细胞在表面上的黏附、增殖和生长。之后，将细胞向成骨谱系分化，以评估碱性磷酸酶（ALP）活性、骨钙素表达和磷酸钙矿化。结果表明，与未改性表面相比，掺杂 Zn 和 Sr 的 NT 表面显著增强了 ADSC 的黏附、增殖、生长和成骨分化，从而证实了这些表面的增强性能。
