Wu Tiantian, Xu Chen, DU Rong, Wen Yang, Chang Jiang, Huan Zhiguang, Zhu Yaqin
Department of General Dentistry, Ninth hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology; National Clinical Research Center of Stomatology.
State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences.
Dent Mater J. 2018 Nov 30;37(6):889-896. doi: 10.4012/dmj.2017-328. Epub 2018 Sep 14.
The objective of this study was to investigate the effects of a silicate-based composite material on proliferation and mineralization of human dental pulp cells (hDPCs), which was compared with those of calcium hydroxide (Ca(OH), CH) and tricalcium silicate (CaSiO, C3S). HDPCs were cultured with CH, C3S and tricalcium silicate/dicalcium silicate (CaSiO/CaSiO, C3S/C2S) composites extract. The CCK-8 assay showed that the composite material stimulated the proliferation of hDPCs. The odontogenic marker genes and DSPP protein expression were more significantly up-regulated by the C3S/C2S composite material compared with pure CH and C3S. HDPCs cultured with composite material extract exert stronger ALP activity and alizarin red S staining. C3S/C2S composite material was advantageous over pure C3S by showing enhanced ability to stimulate the proliferation and odontogenic differentiation of hDPCs, suggesting that the C3S/C2S composite materials possess desirable biocompatibility and bioactivity, and might be a new type of pulp-capping agent and dentin alternative materials.
本研究的目的是探究一种基于硅酸盐的复合材料对人牙髓细胞（hDPCs）增殖和矿化的影响，并将其与氢氧化钙（Ca(OH)₂，CH）和硅酸三钙（Ca₃SiO₅，C₃S）的影响进行比较。将hDPCs与CH、C₃S以及硅酸三钙/硅酸二钙（Ca₃SiO₅/Ca₂SiO₄，C₃S/C₂S）复合材料提取物共同培养。CCK-8检测表明，该复合材料能刺激hDPCs的增殖。与纯CH和C₃S相比，C₃S/C₂S复合材料使牙源性标记基因和DSPP蛋白表达上调更为显著。用复合材料提取物培养的hDPCs表现出更强的碱性磷酸酶（ALP）活性和茜素红S染色。C₃S/C₂S复合材料在刺激hDPCs增殖和牙源性分化方面比纯C₃S更具优势，这表明C₃S/C₂S复合材料具有良好的生物相容性和生物活性，可能是一种新型的盖髓剂和牙本质替代材料。
