Christie B, Musri N, Djustiana N, Takarini V, Tuygunov N, Zakaria M N, Cahyanto A
Faculty of Dentistry, Universitas Padjadjaran, Jalan Sekeloa Selatan 1, Bandung, 40134, Indonesia.
Department of Dental Materials Science and Technology, Faculty of Dentistry, Universitas Padjadjaran, Jalan Raya Bandung Sumedang Km 21, Jatinangor, 45363, Indonesia.
Mater Today Bio. 2023 Sep 23;23:100815. doi: 10.1016/j.mtbio.2023.100815. eCollection 2023 Dec.
Conventional dentistry faces limitations in preserving tooth health due to the finite lifespan of restorative materials. Regenerative dentistry, utilizing stem cells and bioactive materials, offers a promising approach for regenerating dental tissues. Human dental pulp stem cells (hDPSCs) and bioactive materials like calcium phosphate (CaP) and silicate-based materials have shown potential for dental tissue regeneration. This systematic review aims to investigate the effects of CaP and silicate-based materials on hDPSCs through in vitro studies published since 2015. Following the PRISMA guidelines, a comprehensive search strategy was implemented in PubMed MedLine, Cochrane, and ScienceDirect databases. Eligibility criteria were established using the PICOS scheme. Data extraction and risk of bias (RoB) assessment were conducted, with the included studies assessed for bias using the Office of Health and Translation (OHAT) RoB tool. The research has been registered at OSF Registries. Ten in vitro studies met the eligibility criteria out of 1088 initial studies. Methodological heterogeneity and the use of self-synthesized biomaterials with limited generalizability were observed in the included study. The findings highlight the positive effect of CaP and silicate-based materials on hDPSCs viability, adhesion, migration, proliferation, and differentiation. While the overall RoB assessment indicated satisfactory credibility of the reviewed studies, the limited number of studies and methodological heterogeneity pose challenges for quantitative research. In conclusion, this systematic review provides valuable insights into the effects of CaP and silicate-based materials on hDPSCs. Further research is awaited to enhance our understanding and optimize regenerative dental treatments using bioactive materials and hDPSCs, which promise to improve patient outcomes.
由于修复材料的有限寿命，传统牙科在保持牙齿健康方面面临局限性。再生牙科利用干细胞和生物活性材料，为牙齿组织再生提供了一种有前景的方法。人牙髓干细胞（hDPSCs）以及磷酸钙（CaP）和硅酸盐基材料等生物活性材料已显示出牙齿组织再生的潜力。本系统评价旨在通过2015年以来发表的体外研究，调查CaP和硅酸盐基材料对hDPSCs的影响。遵循PRISMA指南，在PubMed MedLine、Cochrane和ScienceDirect数据库中实施了全面的检索策略。使用PICOS方案确定纳入标准。进行了数据提取和偏倚风险（RoB）评估，使用健康与翻译办公室（OHAT）RoB工具对纳入研究的偏倚进行评估。该研究已在OSF注册中心注册。在1088项初始研究中，有10项体外研究符合纳入标准。在所纳入的研究中观察到方法学异质性以及使用了通用性有限的自合成生物材料。研究结果突出了CaP和硅酸盐基材料对hDPSCs活力、黏附、迁移、增殖和分化的积极影响。虽然总体RoB评估表明所审查研究的可信度令人满意，但研究数量有限和方法学异质性给定量研究带来了挑战。总之，本系统评价为CaP和硅酸盐基材料对hDPSCs的影响提供了有价值的见解。期待进一步的研究，以加深我们的理解，并优化使用生物活性材料和hDPSCs的再生牙科治疗，有望改善患者预后。
