香芹酚对大鼠肝脏部分肝切除术后IL-6/STAT3信号通路的影响。

Effect of carvacrol on IL-6/STAT3 pathway after partial hepatectomy in rat liver.

作者信息

Ozen B Durmus, Uyanoglu M

出版信息

Bratisl Lek Listy. 2018;119(9):593-601. doi: 10.4149/BLL_2018_107.

DOI:10.4149/BLL_2018_107

PMID:30226072

Abstract

BACKGROUND

Carvacrol is a component in the essential oil of Lamiaceae family. Beside its antihepatotoxic and hepatoprotective properties it is known to be a contribution to liver regeneration.

OBJECTIVES

This study aimed to investigate the effects of carvacrol in rat liver regeneration after 70 % partial hepatectomy (PHx) through interleukin-6 (IL-6)/signal transducer and activator of transcription 3 (STAT3) and mitogen-activated protein kinases (MAPK) pathways.

METHODS

In our study six groups were formed from 3-month old Wistar albino male rats. Group I and II were Sham operated; Group III and IV received 1 mL 0.9 % NaCl; and Group V and VI were the groups that were treated with 73 mg/kg carvacrol. Group III, IV, V, and VI animals underwent 68-70 % PHx. Dissection was performed 24 and 48 after surgical procedure. Serum Alanine transaminase (ALT) levels were determined. The liver regeneration rate (RR) was calculated. Histopathological analysis was performed by Hematoxylin(et)Eosin staining and the proliferation by proliferating cell nuclear antigen (PCNA) immunohistochemical staining in liver samples. In the liver, certain genes expressions in IL-6/STAT3 and MAPK pathways with RT-PCR analysis and protein expression with Western blot analysis were measured.

CONCLUSION

Carvacrol showed a positive effect on hepatocyte proliferation and liver regeneration 24 and 48 hours after PHx (Tab. 1, Fig. 7, Ref. 39).

摘要

背景

香芹酚是唇形科植物精油的一种成分。除了具有抗肝毒性和肝脏保护特性外，它还对肝脏再生有作用。

目的

本研究旨在通过白细胞介素-6（IL-6）/信号转导子和转录激活子3（STAT3）以及丝裂原活化蛋白激酶（MAPK）途径，研究香芹酚对70%部分肝切除术（PHx）后大鼠肝脏再生的影响。

方法

在我们的研究中，将3个月大的雄性Wistar白化大鼠分为六组。第一组和第二组进行假手术；第三组和第四组接受1 mL 0.9%氯化钠；第五组和第六组用73 mg/kg香芹酚处理。第三组、第四组、第五组和第六组动物接受68 - 70%的PHx。在手术后24小时和48小时进行解剖。测定血清丙氨酸转氨酶（ALT）水平。计算肝脏再生率（RR）。通过苏木精（和）伊红染色进行组织病理学分析，并通过增殖细胞核抗原（PCNA）免疫组织化学染色对肝脏样本中的增殖情况进行分析。在肝脏中，通过逆转录-聚合酶链反应（RT-PCR）分析测定IL-6/STAT3和MAPK途径中某些基因的表达，并通过蛋白质印迹分析测定蛋白质表达。