Laboratory of Drug Discovery and Molecular Engineering, Department of Medicinal Plants, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
National-Regional Joint Engineering Research Center in Hubei for Medicinal Plant Breeding and Cultivation, Huazhong Agricultural University, Wuhan, China.
PLoS One. 2018 Sep 18;13(9):e0203275. doi: 10.1371/journal.pone.0203275. eCollection 2018.
Taraxacum officinale (Asteraceae) is widely distributed weedy plant used as a traditional medicinal herb. The population genetics and historical biogeography of this plant have remained relatively unexplored. This study explores phylogeny, population genetics and ancestral reconstructions adopting multi locus sequence typing (MLST) approach. MLST sequences dataset was generated from genomics and chloroplast DNA sequences obtained from 31 T. officinale haplotypes located in 16 different countries. Phylogenetic analysis distributed these haplotypes in well differentiated geographic clades. The study suggested a close relationship between Europe and adjacent Asian countries. Populations of these regions predominantly formed common haplogroups, showed considerable level of gene flow and evidence for recombination events across European and Asian population. Biogeographical inferences obtained by applying statistical dispersal-vicariance analysis (S-DIVA) and Bayesian binary MCMC (BBM) analysis showed that T. officinale was putatively originated in Europe. Molecular clock analysis based on ITS dataset suggested that the divergence between Europe and East Asian populations can be dated to 1.07 Mya with subsequent dispersal and vicariance events. Among different spatial process long distance seed dispersal mediated by wind had potentially assisted the population expansion of T. officinale.
蒲公英（菊科）是一种广泛分布的杂草植物，被用作传统草药。该植物的群体遗传学和历史生物地理学仍然相对未被探索。本研究采用多位点序列分型（MLST）方法探索了系统发育、种群遗传学和祖先重建。从 31 个蒲公英单倍型位于 16 个不同国家的基因组和叶绿体 DNA 序列中生成了 MLST 序列数据集。系统发育分析将这些单倍型分布在地理上分化良好的地理群中。该研究表明欧洲和邻近的亚洲国家之间存在密切的关系。这些地区的种群主要形成共同的单倍型群，表现出相当水平的基因流和在欧洲和亚洲种群之间发生重组事件的证据。通过应用统计扩散-分歧分析（S-DIVA）和贝叶斯二元 MCMC（BBM）分析进行的生物地理学推断表明，蒲公英可能起源于欧洲。基于 ITS 数据集的分子钟分析表明，欧洲和东亚种群之间的分歧可以追溯到 1.07 Mya，随后发生了扩散和分歧事件。在不同的空间过程中，风介导的远距离种子扩散可能有助于蒲公英种群的扩张。
